1887

Abstract

SUMMARY

The DNA of field isolates and vaccine strains of pseudorabies virus (PRV) was analysed by digestion with the restriction endonuclease HI. A number of distinct restriction profiles of the field isolates obtained from different locations within Europe were observed. As for herpes simplex virus, the variations could be classified into two types: first, alterations in the mobility of fragments due to the presence of additional sequences and/or the occurrence of deletions, a phenomenon most apparent in fragments containing part or whole of the repeat sequence of PRV DNA; second, generation of differently sized fragments due to loss and/or gain of restriction endonuclease cleavage sites. By analysis of several strains with HI a small number of variable cleavage sites were identified within particular regions of the unique long (U) segment of the PRV genome. Compared to wild-type PRV, the restriction fragment patterns of vaccine strains showed characteristic alterations, including the absence of bands, which were non-variable in wild-type strains, and/or the presence of new bands some of which were submolar. Some of these characteristics could be explained by a deletion in the unique short (U) region of the genome of most vaccine strains and the occurrence of closely related variants in the uncloned vaccine virus stocks.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-1-69
1985-01-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/1/JV0660010069.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-1-69&mimeType=html&fmt=ahah

References

  1. Allen G. P., Yeargan M. R., Turtinen L. W., Bryans J. T., McCollum W. H. 1983; Molecular epizootiologic studies of equine herpesvirus-1 infections by restriction endonuclease fingerprinting of viral DNA. American Journal of Veterinary Research 44:263–271
    [Google Scholar]
  2. Bartha A. 1961; Experimental reduction of virulence of Aujeszky’s disease virus. Ungarische Tierärztliche Monatsschrift 16:42–45
    [Google Scholar]
  3. Bartha A., Belak S., Benyeda I. 1969; Trypsin- and heat resistance of some strains of the herpes virus group. Acta veterinaria hungarica 19:97–99
    [Google Scholar]
  4. Baskerville A., McFerran J. B., Dow C. 1973; Aujeszky’s disease in pigs. Veterinary Bulletin 43:465–480
    [Google Scholar]
  5. Ben-Porat T., Veach R. A. 1980; Origin of replication of the DNA of a herpesvirus (pseudorabies). Proceedings of the National Academy of Sciences, U.S.A 77:172–175
    [Google Scholar]
  6. Ben-Porat T., Rixon R. J., Blankenship M. L. 1979; Analysis of the structure of the genome of pseudorabies virus. Virology 95:285–294
    [Google Scholar]
  7. Ben-Porat T., Veach R. A., Ladin B. F. 1980; Replication of herpesvirus DNA. VI. Virions containing either isomer of pseudorabies virus DNA are infectious. Virology 102:370–380
    [Google Scholar]
  8. Ben-Porat T., Deatly A. M., Easterday B. C., Galloway D., Kaplan A. S., McGregor S. 1984; Latency of pseudorabies virus. In Latent Herpes Virus Infections in Veterinary Medicine (Current Topics in Veterinary Medicine and Animal Science, vol. 27) pp 365–383 Edited by Wittmann G., Gaskell R. M., Rziha H. J. Boston & The Hague: Martinus Nijhoff;
    [Google Scholar]
  9. Berns A. J. M., Lai M. H. T., Bosselman R. A., McKennett M. A., Bacheler L. T., Fan H., Robanus Maandag E. C., van Der Putten H., Verma I. M. 1980; Molecular cloning of unintegrated and a portion of integrated Moloney murine leukemia viral DNA in bacteriophage lambda. Journal of Virology 36:254–263
    [Google Scholar]
  10. Buchman T. G., Roizman B., Adams G., Stover B. H. 1978; Restriction endonuclease fingerprinting of herpes simplex DNA: a novel epidemiological tool applied to a nosocomial outbreak. Journal of Infectious Diseases 138:488–498
    [Google Scholar]
  11. Davison A. J., Wilkie N. M. 1981; Nucleotide sequences of the joint between the L and S segments of herpes simplex virus types 1 and 2. Journal of General Virology 55:315–331
    [Google Scholar]
  12. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  13. Engels M., Steck F., Wijler R. 1981; Comparison of the genomes of IBR and IPV virus strains by means of restriction enzyme analysis. Archives of Virology 67:169–174
    [Google Scholar]
  14. Geck P., Nagy E., Lomniczi B. 1982a; Differentiation between Aujeszky’s disease virus strains of different virulence by restriction enzyme analysis of the DNA. Ungarische Tierärztliche Monatsschrift 37:651–656
    [Google Scholar]
  15. Geck P., Medveczky I., Lomniczi B. 1982b; Relation of the Bartha’s vaccine strain and other avirulent Aujeszky’s disease virus strains isolated from field cases. Ungarische Tierärztliche Monatsschrift 37:657–661
    [Google Scholar]
  16. Gielkens A. L. J., Berns A. I. M. 1982; Differentiation of Aujeszky’s disease virus strains by restriction endonuclease analysis of the viral DNAs. In Aujeszky’s Disease (Current Topics in Veterinary Medicine and Animal Science, vol. 17) pp 3–13 Edited by Wittmann G., Hall S. A. Boston & The Hague: Martinus Nijhoff;
    [Google Scholar]
  17. Hayward G. S., Frenkel N., Roizman B. 1975; Anatomy of herpes simplex DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proceedings of the National Academy of Sciences, U.S.A 72:1768–1772
    [Google Scholar]
  18. Herrmann S. C., Heppner B., Ludwig H. 1984; Pseudorabies viruses from clinical outbreaks and latent infections grouped into four major genome types. In Latent Herpes Virus Infections in Veterinary Medicine (Current Topics in Veterinary Medicine and Animal Science, vol. 27) pp 378–401 Edited by Wittmann G., Gaskell R. M., Rziha H. J. Boston & The Hague: Martinus Nijhoff;
    [Google Scholar]
  19. Kilpatrick B. A., Huang E. S., Pagano J. S. 1976; Analysis of cytomegalovirus genomes with restriction endonucleases Hin dIII and Eco RI. Journal of Virology 18:1095–1105
    [Google Scholar]
  20. Ladin B. F., Ihara S., Hampl H., Ben-Porat T. 1982; Pathway of assembly of herpesvirus capsids: an analysis using DNA+ temperature-sensitive mutants of pseudorabies virus. Virology 116:544–561
    [Google Scholar]
  21. Lomniczi B., Blankenship M. L., Ben Porat T. 1984; Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes. Journal of Virology 49:970–979
    [Google Scholar]
  22. Lonsdale D. M., Brown S. M., Subak-Sharpe J. H., Warren K. G., Koprowski H. 1979; The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical and vagus ganglia. Journal of General Virology 43:151–171
    [Google Scholar]
  23. Lonsdale D. M., Brown S. M., Lang J., Subak-Sharpe J. H., Koprowski H., Warren K. G. 1980; Variations in herpes simplex virus isolated from human ganglia and a study of clonal variation in HSV-1. Annals of the New York Academy of Sciences 354:291–308
    [Google Scholar]
  24. Ludwig H., Heppner B., Herrmann D. 1982; The genomes of different field isolates of Aujeszky s disease. In Aujeszky’s Disease (Current Topics in Veterinary Medicine and Animal Science, vol. 17) pp 15–20 Edited by Wittmann G., Hall S. A. Boston & The Hague: Martinus Nijhoff;
    [Google Scholar]
  25. Quint W., Quax W., van Der Putten H., Berns A. 1981; Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues. Journal of Virology 39:1–10
    [Google Scholar]
  26. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  27. Rixon F. J., Ben-Porat T. 1979; Structural evolution of the DNA of pseudorabies defective viral particles. Virology 97:151–163
    [Google Scholar]
  28. Rubenstein A. S., Kaplan A. S. 1975; Electron microscopic studies of the DNA of defective and standard pseudorabies virions. Virology 66:385–392
    [Google Scholar]
  29. Rymo L., Lindahl T., Adams A. 1979; Sites of sequence variability in Epstein-Barr virus DNA from different sources. Proceedings of the National Academy of Sciences, U.S.A 76:2794–2798
    [Google Scholar]
  30. Skoda R., Brauner I., Sadecky E., Mayer V. 1964; Immunisation against Aujeszky’s disease with live vaccine. I. Attenuation of virus and some properties of attenuated strains. Acta virologica 8:1–9
    [Google Scholar]
  31. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  32. Straus S. E., Hay J., Smith H., Owens J. 1983; Genome differences among varicella-zoster isolates. Journal of General Virology 64:1031–1041
    [Google Scholar]
  33. Tatarov G. 1968; Apathogener mutant des Aujeszky-virus induziert von 5-Iodo-2-Deoxyuridin (IUDR). Zentralblatt für Veterinärmedizin 15B:847–853
    [Google Scholar]
  34. van Oirschot J. T., Gielkens A. L. J. 1984a; In vivo and in vitro reactivation of latent pseudorabies virus in pigs bom to vaccinated sows. American Journal of Veterinary Research 45:567–571
    [Google Scholar]
  35. Van Oirschot J. T., Gielkens A. L. J. 1984b; Intranasal vaccination of pigs against pseudorabies. 2. Absence of vaccine virus latency and failure to prevent latency of virulent virus.. American Journal of Veterinary Research (in press)
    [Google Scholar]
  36. Walboomers J. M. M., Ter Schegget J. 1976; A new method for the isolation of herpes simplex vims type 2 DNA. Virology 74:256–258
    [Google Scholar]
  37. Wathen M. W., Pirtle E. C. 1984; Stability of the pseudorabies virus genome after in vivo serial passage. Journal of General Virology 65:1401–1404
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-1-69
Loading
/content/journal/jgv/10.1099/0022-1317-66-1-69
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error