1887

Abstract

SUMMARY

Using immunocytochemical procedures in conjunction with electron microscopy we have examined the distribution of the major DNA-binding protein (DBP) of herpes simplex virus (HSV) in infected nuclei. In embedded specimens, DBP was preferentially associated with fibrillar material of electron-translucent viral inclusions, and to a lesser extent with peripheral (marginated) host chromatin. The latter association was sensitive to a non-ionic detergent (‘Photo flo’). In chromatin spread by the Miller technique, DBP was found to be a component of the 10 nm ‘thick filaments’ previously described in HSV infection.

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1985-01-01
2023-01-27
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References

  1. Bayliss G. J., Marsden H. S., Hay J. 1975; Herpes simplex virus proteins DNA-binding proteins: in infected cells and in the virus structure. Virology 68:124–134
    [Google Scholar]
  2. Bendayan M. 1983; Ultrastructural localization of actin in muscle, epithelial and secretory cells by applying the protein A-gold immunocytochemical technique. Histochemical Journal 15:39–58
    [Google Scholar]
  3. Ben-Ze’Ev A., Abulafia R., Bratosin S. 1983; Herpes simplex virus and protein transport are associated with the cytoskeletal framework and the nuclear matrix in infected BSC-1 cells. Virology 129:501–507
    [Google Scholar]
  4. Beyer A. L., Bouton A. H., Hodge L. D., Miller O. L. 1981; Visualization of the major late R strand transcription unit of adenovirus serotype 2. Journal of Molecular Biology 147:269–295
    [Google Scholar]
  5. Cabral G. A., Courtney R. J., Schaffer P. A., Arciano-Cabral F. 1980; Ultrastructural characterization of an early, nonstructural polypeptide of herpes simplex virus type 1. Journal of Virology 33:1192–1198
    [Google Scholar]
  6. Carlemalm E., Villiger W., Acetarin J. D. 1980; Advances in specimen preparation for electron microscopy. 1 Novel low-temperature embedding resins and a reformulated Vestopal. Experientia 36:740–744
    [Google Scholar]
  7. Conley A. J., Knipe D. M., Jones P. C., Roizman B. 1981; Molecular genetics of herpes simplex virus. VII. Characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in DNA synthesis and accumulation of y polypeptides. Journal of Virology 37:191–206
    [Google Scholar]
  8. Darlington R. W., James C. 1966; Biological and morphological aspects of the growth of equine abortion virus. Journal of Bacteriology 92:250–257
    [Google Scholar]
  9. Dunker R., Brown D. T. 1979; Tubular subviral structure produced in adenovirus-infected KB cells. Journal of Virology 31:568–574
    [Google Scholar]
  10. Dupuy-Coin A. M., Arnoult J., Bouteille M. 1978; Quantitative correlation of morphological alterations of the nucleus with functional events during in vitro infection of glial cells with herpes simplex hominis (HSV 2). Journal of Ultrastructure Research 65:60–72
    [Google Scholar]
  11. Garaud J. C., Eloy R., Moody A. J., Stock C., Grenier J. F. 1980; Glucagon- and glicentin-immunoreactive cells in the human digestive tract. Cell and Tissue Research 213:121–136
    [Google Scholar]
  12. Hay R. T., Hay J. 1981; Characteristics of chromatin preparations from herpes simplex virus infected cells. Biochimica et biophysica acta 655:71–81
    [Google Scholar]
  13. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. Journal of Virology 12:1347–1365
    [Google Scholar]
  14. Kedinger C., Brison O., Perrin F., Wilhelm J. 1978; Structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected HeLa cell nuclei. Journal of Virology 26:364–379
    [Google Scholar]
  15. Knipe D. M., Spang A. E. 1982; Definition of a series of stages in the association of two herpesviral proteins with the cell nucleus. Journal of Virology 43:314–324
    [Google Scholar]
  16. Leinbach S. S., Casto J. F. 1983; Identification and characterization of deoxyribonucleoprotein complexes containing the major DNA-binding protein of herpes simplex virus type 1. Virology 131:274–286
    [Google Scholar]
  17. Leinbach S. S., Summers W. C. 1980; The structure of herpes simplex virus type 1 DNA as probed by micrococcal nuclease digestion. Journal of General Virology 51:45–59
    [Google Scholar]
  18. Littler E., Purifoy D., Minson A., Powell K. L. 1983; Herpes simplex virus non-structural proteins. III,. Function of the major DNA-binding protein. Journal of General Virology 64:983–995
    [Google Scholar]
  19. Matsuguchi M., Puvion-Dutilleul F., Moyne G. 1979; Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures. Journal of General Virology 42:443–456
    [Google Scholar]
  20. Miller O. L., Beatty B. R. 1969; Visualization of nucleolar genes. Science 164:955–957
    [Google Scholar]
  21. Miller O. L., Hodge L. D. 1975; Visualization of a unique nuclear adenovirus DNA-protein complex. Journal of Cell Biology 67:284a
    [Google Scholar]
  22. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  23. Mouttet M-E., Guetard D., Bechet J-M. 1979; Random cleavage of intranuclear herpes simplex virus DNA by micrococcal nuclease. FEBS Letters 100:107–109
    [Google Scholar]
  24. Müller U., Schrӧder C. H., Zentgraf H., Franke W. W. 1980; Coexistence of nucleosomal and various non-nucleosomal chromatin configurations in cells infected with herpes simplex virus. European Journal of Cell Biology 23:197–203
    [Google Scholar]
  25. Okada K., Fujimoto Y., Mikami T. 1974; Electron microscopic autoradiography in the development of herpes virus of turkey. Archiv fur die gesamte Virusforschung 46:111–126
    [Google Scholar]
  26. Powell K. L., Purifoy D. J. M. 1976; DNA-binding proteins of cells infected by herpes simplex virus type 1 and 2. Intervirology 7:225–239
    [Google Scholar]
  27. Powell K. L., Littler E., Purifoy D. J. M. 1981; Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNA-binding protein. Journal of Virology 39:894–902
    [Google Scholar]
  28. Purifoy D. J. M., Powell K. L. 1976; DNA binding proteins induced by herpes simplex virus type 2 in HEp-2 cells. Journal of Virology 19:717–731
    [Google Scholar]
  29. Puvion-Dutilleul F., Puvion E. 1980; New aspects of intranuclear structures following partial decondensation of chromatin: a cytochemical and high-resolution autoradiographical study. Journal of Cell Science 42:305–322
    [Google Scholar]
  30. Puvion-Dutilleul F., Bachellerie J. P., Zalta J. P., Bernhard W. 1977a; Morphology of ribosomal transcription units in isolated subnuclear fractions of mammalian cells. Biologie Cellulaire 30:183–194
    [Google Scholar]
  31. Puvion-Dutilleul F., Bernadac A., Puvion E., Bernhard W. 1977b; Visualization of two different types of nuclear transcriptional complexes in rat liver cells. Journal of Ultrastructure Research 58:108–117
    [Google Scholar]
  32. Puvion-Dutilleul F., Pedron J., Laithier M., Sheldrick P. 1982; Ultrastructural studies on the nucleus of herpes simplex virus type 1-infected cells. Biology of the Cell 44:249–260
    [Google Scholar]
  33. Puvion-Dutilleul F., Pedron J., Cajean-Feroldi C. 1984; Identification of intranuclear structures containing the 72K DNA-binding protein of human adenovirus type 5. European Journal of Cell Biology 34:313–322
    [Google Scholar]
  34. Roth J., Bendayan M., Carlemalm E., Villiger W., Garavito M. 1981; Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. Journal of Histochemistry and Cytochemistry 29:663–671
    [Google Scholar]
  35. Schwartz J., Roizman B. 1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in HEp-2 cells: electron microscopy. Journal of Virology 4:879–889
    [Google Scholar]
  36. Stasiak A., Dicapua E., Roller T. 1983; Unwinding of duplex DNA in complexes with recA protein. Cold Spring Harbor Symposia on Quantitative Biology 47:811–820
    [Google Scholar]
  37. Weller S. K., Lee K. J., Sabourin D. J., Schaffer P. A. 1983; Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein. Journal of Virology 45:354–366
    [Google Scholar]
  38. Wilcox K. W., Kohn A., Sklyanskaya E., Roizman B. 1980; Herpes simplex virus phophoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA. Journal of Virology 33:167–182
    [Google Scholar]
  39. Yeo J., Killington R. A., Watson D. H., Powell K. L. 1981; Studies on cross-reactive antigens in the herpesviruses. Virology 108:256–266
    [Google Scholar]
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