1887

Journal of General Virology header logo

Volume 66, Issue 1

Preliminary Characterization of a Persistent Infection of HeLa Cells with Human Rhinovirus Type 2 Free

Abstract

SUMMARY

We were able to initiate a persistent infection (PI) in HeLa cells with a temperature-sensitive ( ) mutant of rhinovirus type 2 (TS-1), but not with the corresponding wild-type () virus. The ability to initiate a PI may be related to the multiplicity of infection. Persistence was established at 37°C but not at 32°C and the virus isolated from the PI was no longer temperature-sensitive. Infectious virus was continually produced at low levels throughout the course of the PI and cell cultures underwent multiple episodes of partial destruction (crisis) and subsequent recovery. PI virus and the initiating virus were neutralized to the same extent by hyperimmune polyclonal TS-1 antiserum indicating that no significant change had occurred with respect to serological type. The presence of either interferon or virus-related interfering activity could not be demonstrated in the PI cultures. Superinfection experiments in cells that were ‘cured’ of PI virus indicated the selection of a cell population during persistence that could no longer support the growth of homologous-type virus. This effect became less pronounced upon further passage of the cured cells. When compared with the and viruses, the PI virus yielded comparable amounts of infectious virus in HeLa cells but with decreased synthesis of RNA.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HeLa cells , persistent infection and rhinovirus
© Journal of General Virology 1985
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-1-131
1985-01-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/1/JV0660010131.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-1-131&mimeType=html&fmt=ahah

References

  1. Ackermann W. W. 1956; Persistence of poliomyelitis virus during serial passage of HeLa cells. Federation Proceedings 15:580
     [Google Scholar]
  2. Ahmed R., Graham A. F. 1977; Persistent infections in L cells with temperature-sensitive mutants of reovirus. Journal of Virology 23:250–262
     [Google Scholar]
  3. Ahmed R., Canning W. M., Kauffman R. S., Sharpe A. H., Hallum J. V., Fields B. N. 1981; Role of the host Cell in persistent viral infections: coevolution of L cells and reovirus during persistent infection. Cell 25:325–332
     [Google Scholar]
  4. Andzhaparidze O. G., Boriskin Yu. S., Bogomolova N. N., Drynov I. D. 1982; Mumps virus-persistently infected cell cultures release defective interfering virus particles. Journal of General Virology 63:499–503
     [Google Scholar]
  5. Atkins G. J. 1979; Establishment of persistent infection in BHK-21 cells by temperature-sensitive mutants of Sindbis virus. Journal of General Virology 45:201–207
     [Google Scholar]
  6. Came P. E., Schafer T. W., Silver G. H. 1976; Sensitivity of rhinoviruses to human leukocyte and fibroblast interferons. Journal of Infectious Diseases 133: supplement A 136
     [Google Scholar]
  7. Conant R. M., Hamparian V. V. 1968; Rhinoviruses: basis for a numbering system. I. HeLa cells for propagation and serologic procedures. Journal of Immunology 100:107–113
     [Google Scholar]
  8. De B. K., Nayak D. P. 1980; Defective interfering influenza viruses and host cells: establishment and maintenance of persistent influenza virus infection in MDBK and HeLa cells. Journal of Virology 36:847–859
     [Google Scholar]
  9. Evans M. R., Hughes J. H., Gercel C., Hamparian V. V. 1980; Isolation and genetic analysis of temperature sensitive mutants of rhinovirus type 2. Intervirology 13:299–306
     [Google Scholar]
  10. Fisher L. E., Rapp F. 1979; Temperature-dependent expression of measles virus structural proteins in persistently infected cells. Virology 94:55–60
     [Google Scholar]
  11. Friedman R. M., Ramseur J. M. 1979; Mechanisms of persistent infections by cytopathic viruses in tissue culture. Archives of Virology 60:83–103
     [Google Scholar]
  12. Gauntt C. J. 1979; Rhinovirus type 14 persistence in HeLa cells studied by use of guanidine. Journal of Medical Virology 4:115–124
     [Google Scholar]
  13. Gauntt C. J., Griffith M. M., Sauck J. R., Upson R. H., Carlson E. C. 1975; Properties and origins of infectious rhinovirus type 14 particles of different buoyant densities. Journal of Virology 16:1265–1272
     [Google Scholar]
  14. Hamparian V. N. 1979; Rhinoviruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections 5th edn., pp 535–575 Edited by Lennette E. H., Schmidt N. J. Washington, D.C.: American Public Health Association;
     [Google Scholar]
  15. Hodes D. S. 1982; Selection of temperature-sensitive mutants in persistent infection by parainfluenza virus type 3. Journal of General Virology 60:401–404
     [Google Scholar]
  16. Holland J. J., Grabau E. A., Jones C. L., Semler B. L. 1979; Evolution of multiple genome mutations during long-term persistent infection by vesicular stomatitis virus. Cell 16:495–504
     [Google Scholar]
  17. Hughes J. H., Chema S., Lin N., Conant R. M., Hamparian V. V. 1974; Acid lability of rhinovirus: loss of C & D antigenicity after treatment at pH 3.0. Journal of Immunology 112:919–925
     [Google Scholar]
  18. Kennedy J. C., Macdonald R. D. 1982; Persistent infection with infectious pancreatic necrosis virus mediated by defective-interfering (DI) virus particles in a cell line showing strong interference but little DI replication. Journal of General Virology 58:361–371
     [Google Scholar]
  19. McClure M. A., Holland J. J., Perrault J. 1980; Generation of defective interfering particles in picomaviruses. Virology 100:408–418
     [Google Scholar]
  20. May J. D., Menna J. H. 1979; Changes in the virus-host cell relationship in a stable non-virogenic cell line persistently infected with measles virus (BGM/MV). Journal of General Virology 45:185–194
     [Google Scholar]
  21. Meinkoth J., Kennedy S. I. T. 1980; Semliki Forest virus persistence in mouse L929 cells. Virology 100:141–155
     [Google Scholar]
  22. Norval M. 1979; Mechanism of persistence of rubella virus in LLC-MK2 cells. Journal of General Virology 43:289–298
     [Google Scholar]
  23. Preble O. T., Youngner J. S. 1972; Temperature-sensitive mutants isolated from L cells persistently infected with Newcastle disease virus. Journal of Virology 9:200–206
     [Google Scholar]
  24. Preble O. T., Youngner J. S. 1975; Temperature-sensitive viruses and the etiology of chronic and inapparent infections. Journal of Infectious Diseases 131:467–472
     [Google Scholar]
  25. Pringle C. R., Shirodaria P. V., Cash P., Chiswell D. J., Malloy P. 1978; Initiation and maintenance of persistent infection by respiratory syncytial virus. Journal of Virology 28:199–211
     [Google Scholar]
  26. Radloff R. J., Young S. A. 1983; Defective interfering particles of encephalomyocarditis virus. Journal of General Virology 64:1637–1641
     [Google Scholar]
  27. Ramseur J. M., Friedman R. M. 1978; Prolonged infection of L cells with vesicular stomatitis virus: defective interfering forms and temperature-sensitive mutants as factors in the infection. Virology 85:253–261
     [Google Scholar]
  28. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
     [Google Scholar]
  29. Rima B. K., Martin S. I. 1976; Persistent infection of tissue culture cells by RNA viruses. Medical Microbiology and Immunology 162:89–118
     [Google Scholar]
  30. Rowlands D. J., Shirley M. W., Sangar D. V., Brown F. 1975; A high density component in several vertebrate enteroviruses. Journal of General Virology 29:223–234
     [Google Scholar]
  31. Sato H., Ogura H., Hatano M. 1981; An intracellular interaction between a temperature-sensitive mutant and the original wild-type HVJ (Sendai virus) is responsible for the establishment and maintenance of HVJ persistent infection. Journal of General Virology 55:459–468
     [Google Scholar]
  32. Sekellick M. L., Marcus P. I. 1979; Persistent infection. II. Interferon-inducing temperature-sensitive mutants as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 95:36–47
     [Google Scholar]
  33. Takemoto K. K., Habel K. 1959; Virus-cell relationship in a carrier culture of HeLa cells and coxsackie A9 virus. Virology 7:28–44
     [Google Scholar]
  34. Thacore H., Youngner J. S. 1969; Cells persistently infected with Newcastle disease virus. I. Properties of mutants isolated from persistently infected L cells. Journal of Virology 4:244–251
     [Google Scholar]
  35. Wild T. F., Dugre R. 1978; Establishment and characterization of subacute sclerosing panencephalitis (measles) virus persistent infection in BGM cells. Journal of General Virology 39:113–124
     [Google Scholar]
  36. Williams M. P., Brawner T. A., Riggs H. G. Jr, Roehrig J. T. 1981; Characteristics of a persistent rubella infection in a human cell line. Journal of General Virology 52:321–328
     [Google Scholar]
  37. Yamaguchi-koll U., Wiegers K. J., Drzeniek R. 1975; Isolation and characterization of‘dense particles’ from poliovirus-infected HeLa cells. Journal of General Virology 26:307–319
     [Google Scholar]
  38. Youngner J. S., Quagliana D. O. 1976; Temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus. Journal of Virology 19:102–107
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-1-131
Loading
Preliminary Characterization of a Persistent Infection of HeLa Cells with Human Rhinovirus Type 2
J. Gen. Virol. 66, 131 (1985); https://doi.org/10.1099/0022-1317-66-1-131
/content/journal/jgv/10.1099/0022-1317-66-1-131
/content/journal/jgv/10.1099/0022-1317-66-1-131
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error