1887

Abstract

SUMMARY

Two phosphorylated and two non-phosphorylated variants of P protein isolated from Newcastle disease virions are known. Here, a fifth form of P was identified using two-dimensional polyacrylamide gel electrophoresis and peptide mapping. P form 5 was phosphorylated; however, unlike the four known variants of P, the new form was not a major protein in virions, which suggested an intracellular function. The subunit composition of four electrophoretically distinct, disulphide-linked multimers of P from virions was determined. Each homomultimer was composed of at least three molecules of a different one of the four virion-associated P variants.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-9-1631
1984-09-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/9/JV0650091631.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-9-1631&mimeType=html&fmt=ahah

References

  1. Chambers P., Samson A. C. R. 1980; A new structural protein for Newcastle disease virus. Journal of General Virology 50:155–166
    [Google Scholar]
  2. Chinchar V. G., Portner A. 1981; Inhibition of RNA synthesis following proteolytic cleavage of Newcastle disease virus P protein. Virology 115:192–202
    [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  4. Collins P. L., Hightower L. E. 1982; Newcastle disease virus stimulates the cellular accumulation of stress (heat shock) mRNAs and proteins. Journal of Virology 44:703–707
    [Google Scholar]
  5. Collins P. L., Hightower L. E., Ball L. A. 1980; Transcriptional map for Newcastle disease virus. Journal of Virology 35:682–693
    [Google Scholar]
  6. Collins P. L., Fuller F. J., Marcus P. I., Hightower L. E., Ball L. A. 1982; Synthesis and processing of Sindbis virus nonstructural proteins in vitro. Virology 118:363–379
    [Google Scholar]
  7. Colonno R. J., Stone H. O. 1976; Isolation of a transcriptive complex from NDV virions. Journal of Virology 19:1080–1089
    [Google Scholar]
  8. Hamaguchi M., Yoshida T., Nishikawa K., Naruse H., Nagai Y. 1983; Transcriptive complex of Newcastle disease virus. Virology 128:105–117
    [Google Scholar]
  9. Hightower L. E., White F. P. 1981; Cellular responses to stress: comparison of a family of 71–73 kilodalton proteins rapidly synthesized in rat tissue slices and canavanine-treated cells in culture. Journal of Cellular Physiology 108:261–275
    [Google Scholar]
  10. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  11. Madansky C. H., Bratt M. A. 1981; Relationships among virus spread, cytopathogenicity, and virulence as revealed by the noncytopathic mutants of Newcastle disease virus. Journal of Virology 40:691–702
    [Google Scholar]
  12. Morrison T. G. A., Simpson D. 1980; Synthesis, stability, and cleavage of Newcastle disease virus glycoproteins in the absence of glycosylation. Journal of Virology 36:171–180
    [Google Scholar]
  13. O’farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  14. Peeples M. E., Bratt M. A. 1982; UV irradiation analysis of complementation between, and replication of, RNA-negative temperature-sensitive mutants of Newcastle disease virus. Journal of Virology 41:965–973
    [Google Scholar]
  15. Peeples M. E., Rasenas L. L., Bratt M. A. 1982; RNA synthesis by Newcastle disease virus temperature-sensitive mutants in two RNA-negative complementation groups. Journal of Virology 42:996–1006
    [Google Scholar]
  16. Samson A. C. R., Chambers P., Lee C. M., Simon E. 1981; Temperature-sensitive mutant of Newcastle disease virus which has an altered nucleocapsid-associated protein. Journal of General Virology 54:197–201
    [Google Scholar]
  17. Smith G. W., Hightower L. E. 1981a; Biochemical properties of the NDV P protein. In The Replication of Negative Strand Viruses pp 479–484 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier/ North-Holland;
    [Google Scholar]
  18. Smith G. W., Hightower L. E. 1981b; Identification of the P proteins and other disulfide-linked and phosphorylated proteins of Newcastle disease virus. Journal of Virology 37:256–267
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-9-1631
Loading
/content/journal/jgv/10.1099/0022-1317-65-9-1631
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error