Nucleic acid extracted from purified particles of rice gall dwarf virus (RGDV) was identified as double-stranded RNA because it (i) was susceptible to RNase A in 0.1 × standard saline citrate (SSC) but not in 1 × SSC, (ii) was resistant to nucleae S1, (iii) showed no hyperchromicity in u.v. absorption after treatment with formaldehyde at 37 °C, (iv) showed a sharp thermal transition in u.v. absorption in 0.01 × SSC and (v) had a buoyant density of 1.596 g/ml in CsSO. Electrophoresis in polyacrylamide slab gel revealed that RGDV RNA was composed of 12 segments with a total mol. wt. of about 16.9 × 10. Co-electrophoresis of RNA from RGDV and rice dwarf virus demonstrated that the electrophoretic mobilities of the seven larger segments from the two viruses were similar but that the five smaller segments differed in this respect. These results confirm that RGDV is a new virus and a third member of the genus .


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error