1887

Abstract

SUMMARY

Full-length measles virus RNA molecules isolated from purified virions or nucleocapsids and examined by electron microscopy were 5.12(±0.12) µm in length, corresponding to a molecular weight of 5.2(±0.1) × 10. Purified virions examined by negative staining in the electron microscope exhibited a pleomorphic range of particle sizes varying in diameter between 300 nm and 1000 nm. Purified nucleocapsids had dimensions of 21 nm (diameter) × 1254(±7) nm (length) and a central core of diameter about 5 nm. Full-length nucleocapsids were composed of 204(±3) protein discs. The pitch of the nucleocapsid helix was calculated to be 6.1 nm and the helix angle, α, to be 8° 16′. Approximate volume calculations indicate that each enveloped virus particle contains multiple nucleocapsids.

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1984-09-01
2021-10-22
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References

  1. Baczko K., Billeter M., Ter Meulen V. 1983; Purification and molecular weight determination of measles virus genomic RNA. Journal of General Virology 64:1409–1413
    [Google Scholar]
  2. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  3. Dunlap R. C., Milstein J. B., Lundquist M. L. 1983; Identification and characterization of measles virus 50S RNA. Intervirology 19:169–175
    [Google Scholar]
  4. Fraenkel-Conrat H. 1974; Descriptive catalogue of viruses. In Comprehensive Virology vol 1: pp 97–100 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  5. Fraenkel-Conrat H. 1981; Portraits of viruses. Tobacco mosaic virus. Intervirology 15:177–189
    [Google Scholar]
  6. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–188
    [Google Scholar]
  7. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  8. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  9. Lazzarini R. A., Keene J. D., Schubert M. 1981; The origins of defective interfering particles of the negative-strand RNA viruses. Cell 26:145–154
    [Google Scholar]
  10. Miller C. A., Raine C. S. 1979; Heterogeneity of virus particles in measles virus. Journal of General Virology 45:441–453
    [Google Scholar]
  11. Morgan E. M., Rapp F. 1977; Measles virus and its associated diseases. Bacteriological Reviews 41:636–666
    [Google Scholar]
  12. Murant A. F., Taylor M., Duncan G. H., Raschké J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  13. Nakai T., Shand F. L., Howatson A. F. 1969; Development of measles virus in vitro. Virology 38:50–67
    [Google Scholar]
  14. Norrby E. C. J., Magnusson P. 1965; Some morphological characteristics of the internal component of measles virus. Archiv für die gesamte Virusforschung 17:443–447
    [Google Scholar]
  15. Norrby E. C. J., Magnusson P., Falksveden L.-G., Gronberg M. 1964; Separation of measles virus components by equilibrium centrifugation in CsCl gradients. II. Studies on the large and small hemagglutinin. Archiv für die gesamte Virusforschung 14:562–573
    [Google Scholar]
  16. St. Geme J. W. Jr 1964; Evidence for the nucleic acid composition of measles virus. Pediatrics 33:71–74
    [Google Scholar]
  17. Schluederberg A. 1971; Measles virus RNA. Biochemical and Biophysical Research Communications 42:1012–1015
    [Google Scholar]
  18. Sutcliffe J. G. 1979; Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symposia on Quantitative Biology 43:77–90
    [Google Scholar]
  19. Thorne H. V., Dermott E. 1976; Circular and elongated linear forms of measles virus nucleocapsid. Nature, London 264:473–474
    [Google Scholar]
  20. Tucker J., Ramsingh A., Lund G., Scraba D., Leung W.-C., Tyrrell D. L. J. 1983; Characterization of measles virus RNA. In Negative Strand Viruses Edited by Compans R. W., Bishop D. H. L. New York: Academic Press; (in press)
    [Google Scholar]
  21. Tyrrell D. L. J., Norrby E. 1978; Structural polypeptides of measles virus. Journal of General Virology 39:219–229
    [Google Scholar]
  22. Waters D. J., Bussell R. H. 1974; Isolation and comparative study of the nucleocapsids of measles and canine distemper viruses from infected cells. Virology 61:64–79
    [Google Scholar]
  23. Waters D. J., Hersh R. T., Bussell R. H. 1972; Isolation and characterization of measles nucleocapsid from infected cells. Virology 48:278–281
    [Google Scholar]
  24. Waterson A. P. 1965; Measles virus. Archiv für die gesamte Virusforschung 16:57–80
    [Google Scholar]
  25. Waterson A. P., Cruickshank J. G., Lawrence G. P., Hanarek A. P. 1961; The nature of measles virus. Virology 15:379–382
    [Google Scholar]
  26. Ziola B. R., Scraba D. G. 1974; Structure of the Mengo virion. I. Polypeptide and ribonucleate components of the virus particle. Virology 57:531–542
    [Google Scholar]
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