%0 Journal Article %A Gambke, C. %A Senn, H. P. %A Hunsmann, G. %A Schumann, G. %A Moroni, C. %A Alkan, S. S. %T Monoclonal Antibodies Recognizing Structural Components of Murine Retroviruses Including an FMR Antigen on Protein p12 %D 1984 %J Journal of General Virology, %V 65 %N 9 %P 1507-1517 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-65-9-1507 %K monoclonal antibodies %K retrovirus proteins %K FMR antigen %I Microbiology Society, %X SUMMARY Monoclonal antibodies were prepared from mice and rats immunized with Friend leukaemia virus and BALB/c xenotropic virus. By immunoprecipitation of 125I-labelled and [35S]methionine-labelled viruses and by protein blotting, ten antibodies were found to react with the viral components p12, p15, p30, gp70 and p15E/p12E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukaemia viruses (MuLVs). When tested on a panel of ecotropic and xenotropic MuLVs the antibodies revealed the following antigenic specificities: ecotrop-specific on p15E/p12E; xenotrop-specific on p15E; group-specific on p30 and p15E; FM-specific on gp70; FR-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing an FMR determinant localized on p12. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-9-1507