1887

Abstract

SUMMARY

Bovine parvovirus readily produced plaques when inoculated into 60% confluent, actively growing bovine embryonic lung cells. Incorporation of DEAE-dextran, MgCl and DMSO in the agarose overlay medium was found to improve plaque production, especially with the latter chemical. In contrast, protamine sulphate inhibited plaque development. It was found that plaque titration and plaque inhibition tests could be conveniently carried out in 24-well cell culture plates, using an agarose overlay containing DMSO, DEAE-dextran and foetal calf serum. The procedures were highly sensitive, when compared with other established techniques.

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1984-08-01
2022-01-26
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References

  1. Bates R. C., Storz J., Doughri A. M. 1974; Morphogenesis of bovine parvoviruses and associated cellular changes. Experimental and Molecular Pathology 20:208–215
    [Google Scholar]
  2. Hallauer C., Kronauer G. 1965; Extraction of cell-associated virus without damage of the culture. Archivfür die gesamte Virusforschung 15:433–439
    [Google Scholar]
  3. Hierholzer J. C., Suggs M. T., Hall E. C. 1969; Standardised viral haemagglutination and haemagglutination inhibition tests. II. Description and statistical evaluation. Applied Microbiology 18:824–833
    [Google Scholar]
  4. Joo H. S., Donaldson-Wood C. R., Johnson R. H. 1976; A standardised haemagglutination-inhibition test for porcine parvovirus antibody. Australian Veterinary Journal 52:422–424
    [Google Scholar]
  5. Lubeck M. D., Johnson F. B. 1976; Multiplication of bovine parvovirus in two cell strains. Infection and Immunity 13:1289–1292
    [Google Scholar]
  6. Russell P. H. 1979; Malignant catarrhal fever virus – plaque assay and enhanced neutralisation in hypotonic medium. Veterinary Microbiology 4:29–34
    [Google Scholar]
  7. Siegl G. 1976 The Parvoviruses, Virology Monographs vol 15 Edited by Gard S., Hallauer C. Wien & New York: Springer-Verlag;
    [Google Scholar]
  8. Siegl G., Kronauer G. 1980; A plaque assay for feline panleukopenia virus. Journal of General Virology 46:211–218
    [Google Scholar]
  9. Wosu L. O., Johnson R. H., Goodchild I., Bachman P. 1979; Isolation of bovine parvovirus type 1 in Australia. Australian Veterinary Journal 55:199–200
    [Google Scholar]
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