1887

Abstract

SUMMARY

Interferon produced by mouse L-929 cells by incubation with poly(rI).poly(rC) is known to be composed of a mixture of MuIFN- and MuIFN-. The component was separated from the β species by affinity chromatography over a monoclonal anti-MuIFN- agarose column and partially purified by gel filtration. MuIFN-, prepared by this method was separated into at least five subspecies by chromatofocusing. The approximate pI values of these components are ⩾ 7.5, 6.5, 6.2, 5.9 and 5.6, respectively. Component 3 (pI 6.2) was the most prominent subspecies present in our MuIFN- preparations, representing 40 to 50% of the total antiviral activity. Component 1 (pI ⩾ 7.5) which accounted for about 5% of the antiviral activity on mouse cells, differed in some properties from the other interferon subspecies. It showed a relatively high antiviral activity on heterologous cells and it was eluted from a Sephadex column after the other subspecies. Furthermore, it showed a diminished binding to heparin as compared to the other MuIFN- subspecies, indicating a lower affinity for polynucleotides.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-8-1365
1984-08-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/8/JV0650081365.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-8-1365&mimeType=html&fmt=ahah

References

  1. Allen G., Fantes K. H. 1980; A family of structural genes for human lymphoblastoid (leukocyte-type) interferon. Nature, London 287:408–411
    [Google Scholar]
  2. Bosveld I. J., Vonk W. P., Herman R. A. C. P., Van Vliet P. W., De Jonge P., Van Ewijk W., Trapman J. 1982; Preparation and properties of monoclonal antibodies against murine interferon-β. Virology 120:235–239
    [Google Scholar]
  3. Brack C., Nagata S., Mantei N., Weissmann C. 1981; Molecular analysis of the human interferon-α gene family. Gene 15:379–394
    [Google Scholar]
  4. Demaeyer-Guignard J., Thang M. N., Demaeyer E. 1977; Binding of mouse interferon to polynucleotides. Proceedings of the National Academy of Sciences, U,. S,. A 74:3787–3790
    [Google Scholar]
  5. Demaeyer-Guignard J., Tovey M. G., Gresser I., Demaeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly(U)- and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  6. Depomerai D. I., Chesterton C. J., Butterworth P. H. W. 1974; The effect of heparin on the structure and template properties of chromatin. FEBS Letters 42:149–153
    [Google Scholar]
  7. Fuiisawa J. I., Kawade Y. 1981; Properties of nonglycosylated and glycosidase treated mouse L cell interferon species. Virology 112:480–487
    [Google Scholar]
  8. Fujisawa J. I., Iwakura Y., Kawade Y. 1978; Nonglycosylated mouse L cell interferon produced by the action of tunicamycin. Journal of Biological Chemistry 253:8677–8679
    [Google Scholar]
  9. Goeddel D. V., Leung D. W., Dull T. J., Gross M., Lawn R. M., McCandless R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. 1981; The structure of eight distinct cloned human leukocyte interferon cDNA’s. Nature, London 290:20–26
    [Google Scholar]
  10. Havell E. A., Carter W. A. 1981; Effect of tunicamycin on the physical properties and antiviral activities of murine L cell interferon. Virology 108:80–86
    [Google Scholar]
  11. Havell E. A., Spitalny G. L., Patel P. J. 1982; Enhanced production of murine interferon γ by T cells generated in response to bacterial infection. Journal of Experimental Medicine 156:112–127
    [Google Scholar]
  12. Hobbs D. S., Pestka S. 1982; Purification and characterization of interferons from a continuous myeloblastic cell line. Journal of Biological Chemistry 257:4071–4076
    [Google Scholar]
  13. Levy W. P., Rubinstein M., Shively J., Del Valle U., Lai C. Y., Moschera J. J., Brink L., Gerber L., Stein S., Pestka S. 1981; Amino acid sequence of a human leukocyte interferon. Proceedings of the National Academy of Sciences, U,. S,. A 78:6186–6190
    [Google Scholar]
  14. Okamura H., Kawaguchi K., Shoji K., Kawade Y. 1982; High-level induction of γ interferon with various mitogens in mice pretreated with Propionibacterium acnes. Infection and Immunity 38:440–443
    [Google Scholar]
  15. Osborne L. C., Georgiades J. A., Johnson H. M. 1979; Large-scale production and partial purification of mouse immune interferon. Infection and Immunity 23:80–86
    [Google Scholar]
  16. Owerbach D., Rutter W. J., Shows T. B., Gray P., Goeddel D. V., Lawn R. M. 1981; Leukocyte and fibroblast interferon genes are located on human chromosome 9. Proceedings of the National Academy of Sciences, U,. S,. A 78:3123–3127
    [Google Scholar]
  17. Rubinstein M., Levy W. P., Moschera J. A., Lai C. Y., Hershberg R. D., Bartlett R. T., Pestka S. 1981; Human leukocyte interferon: isolation and characterization of several molecular forms. Archives of Biochemistry and Biophysics 210:307–318
    [Google Scholar]
  18. Shaw G. D., Boll W., Taira H., Mantei N., Lengyel P., Weissmann C. 1983; Structure and expression of cloned murine IFN-α genes. Nucleic Acids Research 11:555–573
    [Google Scholar]
  19. Stewart W. E., Havell E. A. 1980; Characterization of a subspecies of mouse interferon cross-reactive on human cells and antigenically related to human leukocyte interferon. Virology 101:315–318
    [Google Scholar]
  20. Trapman J. 1980; Distinct antigenic character of two components of poly(I).poly(C)-induced mouse L cell interferon. FEBS Letters 109:137–140
    [Google Scholar]
  21. Trapman J., Bosveld I. J., Stoof T. J. 1980; Characterization of poly(I).poly(C)-induced mouse L cell interferon. In Interferon: Properties and Clinical Uses pp 183–193 Edited by Khan A., Hill N. O., Dorn G. L. Dallas: Leland Fikes Foundation Press;
    [Google Scholar]
  22. Vonk W. P., Trapman J. 1983; Large-scale, one-step purification of murine interferon-β using a monoclonal antibody. Journal of Interferon Research 3:169–176
    [Google Scholar]
  23. Vonk W. P., HekmaN R. A. C. P., Trapman J. 1981; Properties of poly(I).poly(C)-induced mouse L cell interferon. Virology 113:388–391
    [Google Scholar]
  24. Wietzerbin J., Stefanos S., Lucerno M., Falcoff E., O’Malley J. A., Sulkowski E. 1979; Physico-chemical characterization and partial purification of mouse immune interferon. Journal of General Virology 44:773–781
    [Google Scholar]
  25. Wiranowska-Stewart M., Lin L. S., Braude I. A., Stewart W. E. 1980; Production, partial purification and characterization of human and murine interferon type II. Molecular Immunology 17:625–633
    [Google Scholar]
  26. Yamamoto Y., Kawade Y. 1980; Antigenicity of mouse interferons: distinct antigenicity of the two L cell interferon species. Virology 103:80–88
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-65-8-1365
Loading
/content/journal/jgv/10.1099/0022-1317-65-8-1365
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error