%0 Journal Article %A Hills, R. A. %A McCorkle, G. R. %A Kennedy, D. A. %T Rapid Differentiation of Herpes Simplex Virus Types 1 and 2 by Cytopathic Effect in BS-C-1 Cells %D 1984 %J Journal of General Virology, %V 65 %N 7 %P 1221-1224 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-65-7-1221 %K HSV %K pseudopod projections %K type-specific %I Microbiology Society, %X SUMMARY Conditions are defined under which herpes simplex virus (HSV) type 2 produces a characteristic cytopathic effect (c.p.e.) in African green monkey kidney (BS-C-1) cells. The BS-C-1 microplate c.p.e. test was simultaneously evaluated against immunological and biological marker procedures on 359 HSV strains. A BS-C-1 cell suspension was added to serial dilutions of HSV strains in Falcon Micro Test II plates and incubated at 36.5 °C in a 4% CO2 atmosphere. When the infected cultures were examined microscopically, distinctive pseudopod-like extensions were seen in cultures infected with type 2 strains but not seen when the infecting virus was type 1, forming the basis for a differentiating test. The test was 100% accurate in differentiating between HSV-1 and -2 on a one test per specimen basis. The method is simple, rapid and requires no specialized reagents. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-7-1221