1887

Abstract

SUMMARY

A major virus-specific immediate-early (IE) polypeptide with an apparent mol. wt. of 52000 (52K) was synthesized immediately after the removal of a cycloheximide block applied to owl monkey kidney cell cultures at the time of infection with high multiplicities of herpesvirus saimiri (HVS). The IE 52K polypeptide was phosphorylated and accumulated rapidly and efficiently in the nucleus of infected cells. Monoclonal antibodies to IE 52K and to a major non-structural delayed-early (DE) DNA-binding protein with similar electrophoretic mobility (DE 51K) were used to show that the IE 52K protein and DE 51K proteins are antigenically distinct. The monoclonal antibody to the IE 52K protein of HVS strain 11 reacted in immune precipitation and immunofluorescence tests with polypeptides of similar mol. wt. present as nuclear antigens in cells infected with heterologous strains of HVS and herpesvirus ateles.

Keyword(s): HVS , IE 52K and nuclear phosphoprotein
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-7-1215
1984-07-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/7/JV0650071215.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-7-1215&mimeType=html&fmt=ahah

References

  1. Blair E. D., Honess R. W. 1983; DNA-binding proteins specified by herpesvirus saimiri. Journal of General Virology 64:2697–2715
    [Google Scholar]
  2. Honess R. W., Watson D. H. 1977; Herpes simplex virus resistance and sensitivity to phosphonoacetic acid. Journal of Virology 21:584–600
    [Google Scholar]
  3. Knust E., Dietrich W., Fleckenstein B., Bodemer W. 1983; Virus-specific transcription in a herpesvirus saimiri-transformed lymphoid tumor cell line. Journal of Virology 48:377–383
    [Google Scholar]
  4. Leiden J. M., Buttyan R., Spear P. G. 1976; Herpes simplex virus gene expression in transformed cells. I. Regulation of the viral thymidine kinase gene in transformed L-cells by products of superinfecting virus. Journal of Virology 20:413–424
    [Google Scholar]
  5. MacKem S., Roizman B. 1982; Structural features of the herpes simplex virus α genes 4, 0, and 27 promoter-regulatory sequences which confer a-regulation on chimeric thymidine kinase genes. Journal of Virology 44:939–949
    [Google Scholar]
  6. Marks J. R., Mercer J. A., Spector D. H. 1983; Transcription in mouse embryo cells permissively infected by murine cytomegalovirus. Virology 131:247–254
    [Google Scholar]
  7. Modrow S., Wolf H. 1983; Herpesvirus saimiri-induced proteins in lytically infected cells. I. Time-ordered synthesis. Journal of General Virology 64:37–46
    [Google Scholar]
  8. O’Hare P., Honess R. W. 1983; Identification of a subset of herpesvirus saimiri polypeptides synthesized in the absence of virus DNA replication. Journal of Virology 46:279–283
    [Google Scholar]
  9. Randall R. E., Honess R. W., O’Hare P. 1983; Proteins specified by herpesvirus saimiri: identification and properties of virus-specific polypeptides in productively infected cells. Journal of General Virology 64:19–35
    [Google Scholar]
  10. Reyes G. R., Gavis E. R., Buchan A., Raj N. B. K., Hayward G., Pitha P. 1982; Expression of human a-interferon cDNA under the control of a thymidine kinase promoter from herpes simplex virus. Nature, London 297:598–601
    [Google Scholar]
  11. Roizman B., Kozak M., Honess R. W., Hayward G. S. 1975; Regulation of virus macromolecular synthesis: evidence for multilevel regulation of herpes simplex type 1 RNA and protein synthesis. Cold Spring Harbor Symposia on Quantitative Biology 39:687–695
    [Google Scholar]
  12. Sandri-Goldin R. M., Goldin A. L., Holland L. E., Glorioso J. C., Levine M. 1983; Expression of herpes simplex virus β and γ genes integrated in mammalian cells and their induction by an α gene product. Molecular and Cellular Biology 3:2028–2044
    [Google Scholar]
  13. Stinski M. F., Thomsen D. R., Stenberg R. M., Goldstein L. C. 1983; Organisation and expression of the immediate early genes of human cytomegalovirus. Journal of Virology 46:1–14
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-7-1215
Loading
/content/journal/jgv/10.1099/0022-1317-65-7-1215
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error