1887

Abstract

SUMMARY

The ability of mammalian rotaviruses to replicate in BSC-1 and CV-1 cell cultures was facilitated by the presence of 4% chicken serum. Viral plaques tended to be larger and appear more quickly than in cultures without added chicken serum. It is proposed that chicken serum facilitates plaquing of rotavirus due to its lack of trypsin inhibitors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-7-1207
1984-07-01
2023-02-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/7/JV0650071207.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-7-1207&mimeType=html&fmt=ahah

References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin on the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Barnett B. B., Spendlove R. S., Clark M. K. 1979; Effects of enzymes on rotavirus infectivity. Journal of Clinical Microbiology 10:111–113
    [Google Scholar]
  3. Begin M. E. 1980; Enhanced production of infectious rotavirus in BSC-1 cell cultures by various factors in the presence or absence of trypsin. Journal of General Virology 51:263–270
    [Google Scholar]
  4. Clark S. C., Barnett B. B., Spendlove R. S. 1979; Production of high-titer bovine rotavirus with trypsin. Journal of Clinical Microbiology 9:413–417
    [Google Scholar]
  5. Espejo R. T., Lopez S., Arias C. 1981; Structural polypeptides of simian rotavirus SA-11 and the effect of trypsin. Journal of Virology 37:156–160
    [Google Scholar]
  6. Estes M. K., Graham D. Y. 1979; Enhancement of rotavirus infectivity by trypsin and elastase. INSERM 90:83–86
    [Google Scholar]
  7. Estes M. K., Graham D. Y., Mason B. B. 1981; Proteolytic enhancement of rotavirus infectivity: molecular mechanisms. Journal of Virology 39:879–888
    [Google Scholar]
  8. Fritz H., Trautschold I., Werle E. 1974; Protease inhibitors. In Methods of Enzymatic Analysis vol 2: pp 1066–1071 Edited by Bergmeyer H. U., Gawehn K. New York: Academic Press;
    [Google Scholar]
  9. Fujii M., Tojo H., Koga K., Fukynaga T., Sugimoto Y. 1981; Acid and neutral proteases in the ovarian follicular walls in the domestic fowl Gallus domesticus. Japanese Journal of Zootechnical Science 52:730–735
    [Google Scholar]
  10. Graham D. Y., Estes M. K. 1980; Proteolytic enhancement of rotavirus infectivity: biologic mechanism. Virology 101:432–439
    [Google Scholar]
  11. Smith E. M., Estes M. K., Graham D. Y., Gerba C. P. 1979; A plaque assay for the simian rotavirus SA11. Journal of General Virology 43:513–519
    [Google Scholar]
  12. Zhirnov D. P., Ovcharenko A. V., Bukrinskaya A. G. 1981; Cleavage of influenza virus haemagglutinin by the effect of serum plasmin in cell culture and in vivo. Voprosy virusologii 6:677–687
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-7-1207
Loading
/content/journal/jgv/10.1099/0022-1317-65-7-1207
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error