@article{mbs:/content/journal/jgv/10.1099/0022-1317-65-6-1123, author = "Cubitt, W. D. and Barrett, A. D. T.", title = "Propagation of Human Candidate Calicivirus in Cell Culture", journal= "Journal of General Virology", year = "1984", volume = "65", number = "6", pages = "1123-1126", doi = "https://doi.org/10.1099/0022-1317-65-6-1123", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-6-1123", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "trypsin", keywords = "human", keywords = "calicivirus", abstract = "SUMMARY Evidence is presented for the first time that a human candidate calicivirus (HCV) replicates in human embryo kidney cells when trypsin is incorporated in the culture medium. The virus multiplies in the presence of actinomycin D and radiolabelling experiments with [3H]uridine indicate that it has an RNA genome. These observations provide further support for the view that HCV should be tentatively classified as a member of the Caliciviridae.", }