1887

Abstract

SUMMARY

Pretreatment of HeLa cells with human interferon (IFN) resulted in the inhibition of herpes simplex virus (HSV) replication. We examined the stages in the replication of HSV type 1 and type 2 that were affected by IFN. The rate of synthesis of the HSV immediate-early (α) proteins was inhibited in IFN-pretreated HeLa cells. The subsequent inductions of HSV early () genes, determined by measuring the levels of cytoplasmic mRNA specific for the thymidine kinase, as well as the DNA polymerase enzyme activity, were also suppressed in the IFN-pretreated cells. These results indicate that IFN inhibits HSV replication primarily at a very early step, either prior to, or during the synthesis of α-proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-6-1107
1984-06-01
2024-10-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/6/JV0650061107.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-6-1107&mimeType=html&fmt=ahah

References

  1. Cantell K., Hirvonen S. 1978; Large-scale production of human leukocyte interferon containing 108 units per ml. Journal of General Virology 39:541–543
    [Google Scholar]
  2. Favaloro J., Treisman R., Kamen R. 1980; Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 mapping. Methods in Enzymology 65:718–749
    [Google Scholar]
  3. Garfinke B., McAuslan B. R. 1974; Regulation of herpes simplex virus-induced thymidine kinase. Biochemical and Biophysical Research Communications 58:822–829
    [Google Scholar]
  4. Honess R. W., Roizman B. 1975; Regulation of herpes virus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides. Proceedings of the National Academy of Sciences, U. S. A 72:1276–1280
    [Google Scholar]
  5. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  6. Lengyel P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  7. Lerner M. A., Bailey E. J. 1976; Differential sensitivity of herpes simplex types 1 and 2 to human interferon; antiviral effects of interferon plus 9-β-d-arabinofuranosyladenine. Journal of Infectious Diseases 134:400–404
    [Google Scholar]
  8. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  9. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  10. Nilsen T. W., Maroney P. A., Baglioni C. 1982; Synthesis of (2–5)oligoadenylate and activation of an endonuclease in interferon-treated HeLa cells infected with reovirus. Journal of Virology 42:1039–1045
    [Google Scholar]
  11. Overall J. C., Yeh T. Y., Kern E. R. 1980; Sensitivity of herpes simplex virus types 1 and 2 to three preparations of human interferon. Journal of Infectious Diseases 142:943
    [Google Scholar]
  12. Panet A., Falk H. 1983; Inhibition by interferon of herpes simplex virus thymidine kinase and DNA polymerase in infected and biochemically transformed cells. Journal of General Virology 64:1999–2006
    [Google Scholar]
  13. Pereira L., Wolff M. H., Fenwick M., Roizman B. 1977; Regulation of herpes virus macromolecular synthesis: properties of α-polypeptides made in HSV-1 and HSV-2 infected cells. Virology 77:733–749
    [Google Scholar]
  14. Powell K., Purifoy D. J. M. 1977; Nonstructural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase. Journal of Virology 24:618–626
    [Google Scholar]
  15. Purifoy D. J. M., Benyesh-Melnick M. 1975; DNA polymerase induction by DNA-negative temperature-sensitive mutants of herpes simplex virus type-2. Virology 68:374–386
    [Google Scholar]
  16. Rasmussen L., Farley L. 1975; Inhibition of herpes virus hominis replication by human interferon. Infection and Immunity 12:104–108
    [Google Scholar]
  17. Read G. S., Frenkel N. 1983; Herpes simplex virus mutants defective in the virion-associated shut-off of host polypeptide synthesis and exhibiting abnormal synthesis of α (immediate-early) viral polypeptides. Journal of Virology 46:498–512
    [Google Scholar]
  18. Silverman R. H., Cayley P. J., Knight M., Gilbert C. S., Kerr I. M. 1982; Control of the ppp(A2′ p)nA system in HeLa cells: effects of interferon and virus infection. European Journal of Biochemistry 124:131–138
    [Google Scholar]
  19. Stinski M. F., Thomsen D. R., Rodriguez J. E. 1982; Synthesis of human cytomegalovirus-specified RNA and protein in interferon-treated cells at early times after infection. Journal of General Virology 60:261–270
    [Google Scholar]
  20. Whitaker-Dowling P. A., Wilcox D. K., Widnell C. C., Youngner J. S. 1983; Interferon-mediated inhibition of virus penetration. Proceedings of the National Academy of Sciences, U. S. A 80:1083–1086
    [Google Scholar]
  21. Wigler M., Silverstein S., Lee L. S., Pellicer A., Cheng Y. C., Axel R. 1977; Transfer of purified herpes virus thymidine kinase gene into mouse cells. Cell 11:223–232
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-65-6-1107
Loading
/content/journal/jgv/10.1099/0022-1317-65-6-1107
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error