RT Journal Article SR Electronic(1) A1 Brown, S. Morira A1 Harland, June A1 Subak-Sharpe, J. H.YR 1984 T1 Isolation of Restriction Endonuclease Site Deletion Mutants of Herpes Simplex Virus JF Journal of General Virology, VO 65 IS 6 SP 1053 OP 1068 DO https://doi.org/10.1099/0022-1317-65-6-1053 PB Microbiology Society, SN 1465-2099, AB SUMMARY We provide evidence that (i) variants lacking individual herpes simplex virus type 1 (HSV-1) XbaI sites can be selected following extensive XbaI treatment of the viral DNA and can be recombined to produce HSV-1 variants lacking two of the four sites normally found, (ii) all XbaI sites can be removed from a viable intertypic recombinant HSV genome, (iii) following XbaI treatment, different mutants with deletions (0.15 to 8.8 kb) in the long repeat (TRL or IRL) and long unique regions can be readily isolated, as well as mutants with novel XbaI sites, (iv) several mutants with deletions in one of the repeats (TRL or IRL) have a measurable growth disadvantage in tissue culture., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-6-1053