1887

Abstract

SUMMARY

A phage-resistant mutant of (strain YIT 9021) was capable of adsorbing both PL-1 and J-1 phages, but did not yield phage-infected cells. The mutant and wild-type strains were identical in morphology and sugar composition of the cell walls. Attempts to induce prophages from strain YIT 9021 were unsuccessful. Electron microscopic examination of negatively stained mixtures of phage PL-1 and YIT 9021 bacteria revealed that the phages were adsorbed to the cells in a tail-first orientation. All the adsorbed phages had DNA-filled heads. It was concluded that PL-1 adsorbed normally but was blocked in the injection of the phage genome into the cell.

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1984-05-01
2024-04-19
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References

  1. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  2. Chen P. S. Jr, Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  3. Hadźija O. 1974; A simple method for the quantitative determination of muramic acid. Analytical Biochemistry 60:512–517
    [Google Scholar]
  4. Hino M., Ikebe N. 1965; Studies on the lactic acid bacteria employed for beverage production. II. Isolation and some properties of a bacteriophage infected during the fermentation of lactic acid beverage. Nippon Nogeikagaku Kaishi 39:472–476
    [Google Scholar]
  5. Ishibashi K., Takesue S., Watanabe K., Oishi K. 1982; Use of lectins to characterize the receptor sites for bacteriophage PL-1 of Lactobacillus casei. Journal of General Microbiology 128:2251–2259
    [Google Scholar]
  6. Kanegasaki S., Tomita T. 1976; Mutants of Salmonella anatum that block bacteriophage ε15 infection at early stages. Journal of Bacteriology 127:7–13
    [Google Scholar]
  7. Kohsaka T. 1977; Physicochemical properties of a virulent Lactobacillus phage containing DNA with cohesive ends. Journal of General Virology 37:209–214
    [Google Scholar]
  8. Lwoff A., Siminovitch L., Kjeldgaard N. 1950; Induction de la lyse bacteriophagique de la totalité d’une population microbienne lysogene. Compte rendu hebdomadaire des séances de l’Académie des sciences 231:190–191 English translation in Stent G. S., Papers on Bacterial Viruses, p. 332
    [Google Scholar]
  9. Otsuji N., Sekiguchi M., Iuima T., Takagi Y. 1959; Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Nature, London 184:1079–1080
    [Google Scholar]
  10. Scandella D., Arber W. 1974; An Escherichia coli mutant which inhibits the injection of phage λ DNA. Virology 58:504–513
    [Google Scholar]
  11. Watanabe K., Takesue S. 1972; The requirement for calcium in infection with Lactobacillus phage. Journal of General Virology 17:19–30
    [Google Scholar]
  12. Watanabe K., Takesue S. 1973; Energy requirement for the formation of blender-resistant complexes in Lactobacillus phage infection. Journal of General Virology 20:319–326
    [Google Scholar]
  13. Watanabe K., Takesue S. 1975; Use of L-rhamnose to study irreversible adsorption of bacteriophage PL–1 to a strain of Lactobacillus casei. Journal of General Virology 28:29–35
    [Google Scholar]
  14. Watanabe K., Takesue S., Jin-Nai K., Yoshikawa T. 1970; Bacteriophage active against the lactic acid beverage-producing bacterium Lactobacillus casei. Applied Microbiology 20:409–415
    [Google Scholar]
  15. Watanabe K., Takesue S., Ishibashi K. 1979; Adenosine triphosphate content in Lactobacillus casei and the blender-resistant phage-cell complex-forming ability of cells on infection with PL-1 phage. Journal of General Virology 42:27–36
    [Google Scholar]
  16. Watanabe K., Takesue S., Ishibashi K., Iwamoto T., Kondo J. 1982; Fine structure of PL-1 phage active against Lactobacillus casei. Journal of General and Applied Microbiology 28:1–6
    [Google Scholar]
  17. Yokokura T., Kodaira S., Ishiwa H., Sakurai T. 1974; Lysogeny in lactobacilli. Journal of General Microbiology 84:277–284
    [Google Scholar]
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