1887

Abstract

SUMMARY

Infection of L929 or BW5147 cells with mumps virus was shown to result in an abortive type of infection in that little or no progeny virus was produced. Both cell lines could adsorb mumps virus, indicating that restricted viral growth in these cells was not attributable to the absence of mumps virus receptors. Using [S]methionine-labelled virus, it was demonstrated that restriction of virus growth in BW5147 cells was due in part to inefficient virus penetration into the cells. Virus-specific polypeptides were synthesized in mumps virus-infected L929 cells but were not detected in infected BW5147 cells. After addition of actinomycin D or anti-interferon serum to the cultures, mumps virus was able to replicate in L929 cells, whereas no virus growth was apparent in BW5147 cells.

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/content/journal/jgv/10.1099/0022-1317-65-5-973
1984-05-01
2022-01-17
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References

  1. Ball L. A. 1979; Induction of 2′, 5′-oligoadenylate synthetase activity and a new protein by chick interferon. Virology 94:282–296
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Fujita T., Saito S., Kohno S. 1979; Priming increases the amount of interferon mRNA in poly(rI).poly(rC)-treated L cells. Journal of General Virology 45:301–308
    [Google Scholar]
  4. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  5. Nagai Y., Ito Y., Hamaguchi M., Yoshida T., Matsumoto T. 1981; Relation of interferon production to the limited replication of Newcastle disease virus in L cells. Journal of General Virology 55:109–116
    [Google Scholar]
  6. Toyama S., Toyama S., Uetake H. 1977; Altered cell-fusion capacity in lines of KB cells resistant to Sendai virus-induced cytolysis. Virology 76:503–515
    [Google Scholar]
  7. Tsurudome M., Yamada A., Hishiyama M., Ito Y. 1983; Replication of mumps virus in various cells. Archives of Virology (in press)
    [Google Scholar]
  8. Whitaker-dowling P. A., Wilcox D. K., Windell C. C., Youngner J. S. 1983; Interferon-mediated inhibition of virus penetration. Proceedings of the National Academy of Sciences, U. S. A 80:1083–1086
    [Google Scholar]
  9. Youngner J. S., Scott A. W. 1968; Relation of interferon synthesis to abortive replication of Newcastle disease virus in L cells. Journal of Virology 2:810–82
    [Google Scholar]
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