RT Journal Article SR Electronic(1) A1 Theilmann, D. A1 Eaton, B. T. A1 Downe, A. E. R.YR 1984 T1 Properties of Sindbis Virus Variants from Infected Culex tarsalis Mosquitoes JF Journal of General Virology, VO 65 IS 5 SP 945 OP 953 DO https://doi.org/10.1099/0022-1317-65-5-945 PB Microbiology Society, SN 1465-2099, AB SUMMARY Plaque-purified Sindbis virus was passed three times in Culex tarsalis mosquitoes and progeny viruses were isolated by plaque purification on a cloned line of Aedes albopictus cells. Nine of ten clones examined differed from wild-type (wt) virus with respect to their plaque morphology characteristics in chick embryo fibroblast (CEF) and/or A. albopictus cells. Seven clones were temperature-sensitive and failed to replicate or synthesize viral RNA in CEF cells at 41 °C. At 35 °C in CEF cells the majority of isolates synthesized less viral RNA than wt virus. In contrast, all cloned isolates synthesized viral RNA more rapidly than wild-type virus in A. albopictus cells., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-5-945