1887

Abstract

SUMMARY

Plaque-purified Sindbis virus was passed three times in mosquitoes and progeny viruses were isolated by plaque purification on a cloned line of cells. Nine of ten clones examined differed from wild-type (wt) virus with respect to their plaque morphology characteristics in chick embryo fibroblast (CEF) and/or cells. Seven clones were temperature-sensitive and failed to replicate or synthesize viral RNA in CEF cells at 41 °C. At 35 °C in CEF cells the majority of isolates synthesized less viral RNA than wt virus. In contrast, all cloned isolates synthesized viral RNA more rapidly than wild-type virus in cells.

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1984-05-01
2022-01-25
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