We have previously reported that among a series of human tumours investigated, only human teratocarcinoma cell lines derived from testicular tumours or pulmonary metastases of patients in Germany and the U.S.A. produced retrovirus-like particles spontaneously, albeit in low amounts. In a recent publication electron microscopical data suggested that the human teratocarcinoma-derived (HTD) particles were morphologically closely related, but not identical, to the type C retroviruses of animals. In this communication, the explantation of three human teratocarcinoma cell lines is briefly described. Evidence is presented that HTD particles (i) are synthesized only in a fraction of the epithelioid and differentiating cells; (ii) can be induced biochemically in a manner characteristic of retroviruses; (iii) either are not infectious or possess a peculiar host range; (iv) are immunologically unrelated to animal retrovirus strains; (v) possess an endogenous RNA-dependent DNA polymerase activity that can be banded at 1.16 g/ml in linear sucrose gradients. These results may be taken as suggestive evidence that HTD particles represent a novel group of unique retroviruses.
AhmedM.,
SchidlovskyG.,
KorolW.,
VidrineG.,
CicmanecJ. L.1974; Occurrence of Mason-Pfizer monkey virus in healthy rhesus monkeys. Cancer Research 34:3504–3508
BarbacidM.,
BolognesiD. P.,
AaronsonS. A.1980; Humans have antibodies capable of recognizing oncoviral glycoproteins: demonstration that these antibodies are formed in response to cellular modification of glycoproteins rather than as consequence of exposure to virus. Proceedings of the National Academy of Sciences, U.S.A 77:1617–1621
BenvenisteR. E.,
LieberM. M.,
LivingstonD. M.,
SherrC. J.,
TodaroG. J.,
KalterS. S.1974; Infectious C- type virus isolated from a baboon placenta. Nature, London 248:17–20
BollerK.,
FrankH.,
LöwerJ.,
LöwerR.,
KurthR.1983; Structural organization of unique retrovirus-like particles budding from human teratocarcinoma cell lines. Journal of General Virology 64:2549–2559
BronsonD. L.,
RitziD. M.,
FraleyE. E.,
DaltonA. J.1978; Morphologic evidence for retrovirus production by epithelial cells derived from a human testicular tumor metastasis. Journal of the National Cancer Institute 60:1305–1308
BronsonD. L.,
FraleyE. E.,
FoghJ.,
KalterS. S.1979; Induction of retrovirus particles in human testicular tumor (Tera-1) cell cultures: an electron microscopic study. Journal of the National Cancer Institute 63:337–339
DaltonA. J.,
HellmannA.,
KalterS. S.,
HelmkeR. J.1974; Ultrastructural comparison of placental virus with several type-C oncogenic viruses. Journal of the National Cancer Institute 52:1379–1381
DemseyA.,
CollinsF.,
KawkaD.1980; Structure of and alterations to defective murine sarcoma virus particles lacking envelope proteins and polyprotein cleavage. Journal of Virology 36:872–877
DirksenE. R.,
LevyJ. A.1977; Virus-like particles in placentas from normal individuals and patients with systemic lupus erythematosus. Journal of the National Cancer Institute 59:1187–1192
FrankH.,
SchwarzH.,
GrafT.,
SchäferW.1978; Properties of mouse leukemia viruses. XV. Electron microscopic studies on the organization of Friend leukemia virus and other mammalian C-type viruses. Zeitschrift fur Naturfor schung 33c:124–138
GalloR. C.,
SaxiW. C.,
GallagherR. E.,
GillespieD. H.,
RuscettiF.,
ReitzM. S.,
AulakhG. S.,
Wong-StaalF.1977; Some ideas on the origin of leukemia in man and recent evidence for the presence of acquired and endogenous type-C viral related sequences. In Origins of Human Cancer pp 1253–1285 Edited by
HiattH. H.,
WatsonJ. D.,
WinstenJ. A.
New York: Cold Spring Harbor Laboratory;
GardnerM. B.1971; Current information on feline and canine cancers and relationship or lack of relationship to human cancer. Journal of the National Cancer Institute 46:281–290
GardnerM. B.,
RasheedS.,
ShimizuS.,
RongeyR. W.,
HendersonB. E.,
McallisterR. M.,
KlementV.,
CharmanH. P.,
GildenR. V.,
HeberlingR. L.,
HuebnerR. J.1977; Search for RNA tumor virus in humans. In Origins of Human Cancer pp 1235–1251 Edited by
. HiattH. H.,
WatsonJ. D.,
WinstenJ. A.
New York: Cold Spring Harbor Laboratory;
GrossL.,
SchidlovskyG.,
FeldmanD.,
DreyfussY.,
MooreL. A.1975; C-type virus particles in placenta of normal healthy Sprague-Dawley rats. Proceedings of the National Academy of Sciences, U.S.A 72:3240–3244
HinumaY.,
NagataK.,
HanaokaM.,
NakaiM.,
MatsumotoT.,
KinoshitaK.-J.,
SharakawaS.,
MiyoshiI.1981; Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proceedings of the National Academy of Sciences, U.S.A 78:6476–6480
HirshautY.,
PeiD. T.,
MarcoveR. C.,
MukherjiB.,
Spiel VogelA. R.,
EssnerE.1974; Seroepidemiology of human sarcoma antigen (S,). New England Journal of Medicine 291:1103–1107
ImamuraM.,
PhillipsP. E.,
MellorsR. C.1976; The occurrence and frequency of type C virus-like particles in placentas from patients with systemic lupus erythematosus and from normal subjects. American Journal of Pathology 83:383–394
KalterS. S.,
HelmkeR. J.,
HeberlingR. L.,
PanigelM.,
FowlerA. K.,
StricklandJ. E.,
HellmannA.1973a; C-type particles in normal human placentas. Journal of the National Cancer Institute 50:1081–1084
KalterS. S.,
HeberlingR. L.,
SmithC. C.,
HelmkeR. J.1975; C-type viruses in chimpanzee (Pan sp.) placentas. Journal of the National Cancer Institute 55:735–736
KurthR.,
MikschyU.1978; Human antibodies reactive with purified envelope antigens of primate type C tumor viruses. Proceedings of the National Academy of Sciences, U.S.A 75:5692–5696
KurthR.,
TeichN. M.,
WeissR.,
OliverR. T. D.1977; Natural human antibodies reactive with primate type-C viral antigens. Proceedings of the National Academy of Sciences, U.S.A 74:1237–1241
KurthR.,
LöwerR.,
LöwerJ.,
HarzmannR.,
PfeifferR.,
SchmidtC. G.,
FoghJ.,
FrankH.1980; Oncovirus synthesis in human teratocarcinoma cultures and an increased anti-viral immune reactivity in corresponding patients. In Viruses in Naturally Occurring Cancers pp 835–846 Edited by
EssexM.,
TodaroG. J.,
zur HausenH.
New York: Cold Spring Harbor Laboratory;
LöwerJ.,
DavidsonE. A.,
TeichN. M.,
WeissR. A.,
JosephA. P.,
KurthR.1981a; Heterophil human antibodies recognize oncovirus envelope antigens: epidemiological parameters and immunological specificity of the reaction. Virology 109:409–417
LöwerJ.,
LöwerR.,
StegmannJ.,
FrankH.,
KurthR.1981b; Retrovirus particle production in three of four human teratocarcinoma cell lines. In Modem Trends in Human Leukemia vol 4 pp 541–544 Edited by
NethR.,
GalloR. C.,
GrafT.,
MannweilerK.
Berlin: Springer-Verlag;
NelsonJ. A.,
LevyJ. A.,
LeongJ. C.1981; Human placentas contain a specific inhibitor of RNA-directed DNA polymerase. Proceedings of the National Academy of Sciences, U.S.A 78:1670–1674
PoieszB. J.,
RuscettiF. W.,
GazdarA. F.,
BunnP. A.,
MinnaJ. D.,
GalloR. C.1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences. U.S.A 77:7415–7419
RamsayG.,
HaymanM. J.1980; Analysis of cells transformed by defective leukemia virus OK 10: production of non-infectious particles and synthesis of Pr76gag and an additional 200000 dalton protein. Virology 106:71–81
ReitzM. S.,
PoieszB. J.,
RuscettiF. W.,
GalloR. C.1981; Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes. Proceedings of the National Academy of Sciences, U.S.A 78:1887–1891
RokutandaM.,
MaedaY. Y.,
WatanabeS. T.1982; An inhibitor of reverse transcriptase in the cytoplasm of the cultured cells and the spleen of mice. Biochemical and Biophysical Research Communications 108:510–516
SchidlovskyG.,
AhmadM.1973; C-type virus particles in placentas and fetal tissues of rhesus monkeys. Journal of the National Cancer Institute 51:225–233
SemanG.,
LevyB. M.,
PanigelM.,
DmochowskiL.1975; Type-C virus particles in placenta of the cottontop marmoset (Saguinus oedipus). Journal of the National Cancer Institute 54:251–252
ShikuH.,
TakahashiT.,
ResnickL. A.,
OettgenH. F.,
OldL. J.1977; Cell surface antigens of human malignant melanoma. II. Recognition of autoantibodies with unusual characteristics. Journal of Experimental Medicine 145:784–789
SmithG. C.,
KalterS. S.,
HelmkeR. J.,
HeberlingR. L.,
PanigelM.,
KraemerD. C.1975; A-type particles in placentas of four mouse strains. Proceedings of the Society for Experimental Biology and Medicine 148:1212–1216
SmithG. C.,
HeberlingR. L.,
HelmkeR. J.,
BarberS. T.,
KalterS. S.1977; Oncornavirus-like particles in squirrel monkey (Saimiri sciureus) placenta and placenta culture. Journal of the National Cancer Institute 59:975–979
SnyderH. W.Jr,
FleissnerE.1980; Specificity of human antibodies to oncovirus glycoproteins: recognition of antigen by natural antibodies directed against carbohydrate structures. Proceedings of the National Academy of Sciences, U.S.A 77:1622–1626
TodaroG. J.,
BenvenisteR. E.,
SherwinS. A.,
SherrC. J.1978; MAC-1, a new genetically transmitted type-C virus of primates: ‘low frequency’ activation from stumptail monkey cell cultures. Cell 13:775–782
VernonM. L.,
McMahonJ. M.,
HackettJ. J.1974; Additional evidence of type C particles in human placentas. Journal of the National Cancer Institute 52:987–989
WernickeD.,
KurthR.1981; Human antibodies recognizing the envelope glycoprotein of the baboon endogenous virus BaEV are of heterophil origin. Medical Microbiology and Immunology 170:135–143