%0 Journal Article %A Davison, M.-J. %A Preston, V. G. %A McGeoch, D. J. %T Determination of the Sequence Alteration in the DNA of the Herpes Simplex Virus Type 1 Temperature-sensitive Mutant ts K %D 1984 %J Journal of General Virology, %V 65 %N 5 %P 859-863 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-65-5-859 %K HSV-1 ts K %K marker rescue %K Vmw 175 %I Microbiology Society, %X SUMMARY The alteration in the DNA sequence responsible for the mutant phenotype of the herpes simplex virus type 1 temperature-sensitive mutant ts K has been determined. A single C:G base pair present in the wild-type Vmw 175 immediate-early polypeptide-coding sequence has been replaced by T:A in the mutant gene. This results in the substitution of an alanine by a valine codon. In two revertants the mutant T:A base pair has been resubstituted by C:G. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-5-859