RT Journal Article SR Electronic(1) A1 Tomley, Fiona M. A1 Cooper, R. J.YR 1984 T1 The DNA Polymerase Activity of Vaccinia Virus ‘Virosomes’: Solubilization and Properties JF Journal of General Virology, VO 65 IS 4 SP 825 OP 829 DO https://doi.org/10.1099/0022-1317-65-4-825 PB Microbiology Society, SN 1465-2099, AB Summary Intracellular DNA-protein complexes (‘virosomes’) of vaccinia virus have been isolated. The solubilization of the ‘virosome’-bound DNA polymerase activity was attempted by a variety of high-salt extraction procedures. The most efficient of these used 0.3 m-ammonium sulphate followed by brief sonication. The solubilized DNA polymerase activity from the ‘virosomes’, together with the DNA polymerases from 100000 g supernatant fluids from the cytoplasm of infected and uninfected cells were chromatographed on DEAE-cellulose and their properties compared. The ‘virosome’ DNA polymerase activity differed from the soluble vaccinia virus-induced DNA polymerase activity in its requirements for divalent cations and in respect of pH optimum, K m for the deoxyribonucleoside triphosphates and the effect of N-ethylmaleimide., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-4-825