1887

Abstract

SUMMARY

A procedure is presented for isolating the nucleocapsid proteins, N and NP from vesicular stomatitis virus and Sendai virus respectively, in soluble form. These proteins were suitable for the determination of their blocked amino-terminal peptide sequences by gas-liquid chromatography/mass spectrometry at the low nanomole level. The N protein prepared by this procedure was previously shown to retain some of its expected biological activity. The sequence of 626 nucleotides from the 3′ end of the Sendai virus genome, which includes the first one-third of the NP gene, was determined. Using this information, primer extension studies on intracellular Sendai virus mRNAs allowed the determination of the structure of the leader-NP intervening sequence and the 5′ end of the NP mRNA. Comparison of the amino termini of the nucleocapsid proteins with their respective mRNA sequences revealed that these proteins are similarly processed .

Keyword(s): nucleocapsid , Sendai virus and VSV
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1984-04-01
2021-10-21
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References

  1. Banerjee A. K., Abraham G., Colonno R. J. 1977; Vesicular stomatitis virus: mode of transcription. Journal of General Virology 34:1–8
    [Google Scholar]
  2. Blumberg B. M., Kolakofsky D. 1983; An analytical review of defective infections of vesicular stomatitis virus. Journal of General Virology 64:1839–1847
    [Google Scholar]
  3. Blumberg B. M., Giorgi C., Kolakofsky D. 1983; N protein of vesicular stomatitis virus selectively encapsidates leader RNA in vitro. Cell 32:559–567
    [Google Scholar]
  4. Dowling P. C., Giorgi C., Roux L., Dethlefsen L. A., Galantowicz M. E., Blumberg B. M., Kolakofsky D. 1983; Molecular cloning of the 3′-proximal third of Sendai virus genome. Proceedings of the National Academy of Sciences, U. S. A 80:5213–5216
    [Google Scholar]
  5. Emerson S. U., Yu Y.-H. 1975; Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. Journal of Virology 15:1348–1356
    [Google Scholar]
  6. Gallione C. J., Greene J. R., Iverson L. E., Rose J. K. 1981; Nucleotide sequences of the mRNAs encoding the vesicular stomatitis virus N and NS proteins. Journal of Virology 39:529–535
    [Google Scholar]
  7. Giorgi C., Blumberg B. M., Kolakofsky D. 1983a; Sequence determination of the (+) leader RNA regions of the vesicular stomatitis virus Chandipura, Cocal and Piry serotype genomes. Journal of Virology 46:125–130
    [Google Scholar]
  8. Giorgi C., Blumberg B. M., Kolakofsky D. 1983b; Sendai virus contains overlapping genes expressed from a single mRNA. Cell 35:829–836
    [Google Scholar]
  9. Gray W. R., Del Valle U. E. 1970; Application of mass spectrometry to protein chemistry. I. Method for amino-terminal sequence analysis of proteins. Biochemistry 9:2134–2137
    [Google Scholar]
  10. Heggeness M. H., Scheid A., Choppin P. W. 1981; The relationship of conformational changes in the Sendai virus nucleocapsid to proteolytic cleavage of the NP polypeptide. Virology 114:555–562
    [Google Scholar]
  11. Keene J. D., Shubert M., Lazzarini R. A. 1980; Intervening sequence between the leader region and the nucleocapsid gene of vesicular stomatitis virus RNA. Journal of Virology 33:789–794
    [Google Scholar]
  12. Keene I. D., Thornton J., Emerson S. U. 1981; Sequence specific contacts between the RNA polymerase of VSV and the leader gene. Proceedings of the National Academy of Sciences, U. S. A 78:6192–6195
    [Google Scholar]
  13. Kingsbury D. W. 1974; The molecular biology of paramyxoviruses. Medical Microbiology and Immunology 160:73–83
    [Google Scholar]
  14. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  15. Kurilla M. G., Keene J. D. 1983; The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies. Cell 34:837–845
    [Google Scholar]
  16. Kurilla M. G., Piwnica-Worms H., Keene J. D. 1982; Rapid and transient localization of the leader RNA of VSV in the nuclei of infected cells. Proceedings of the National Academy of Sciences, U. S. A 79:5240–5244
    [Google Scholar]
  17. Lamb R. A., Choppin P. W. 1977; The synthesis of Sendai virus polypeptides in infected cells. Virology 81:382–397
    [Google Scholar]
  18. Lamb R. A., Mahy B. W. J. 1975; The polypeptides and RNA of Sendai virus. In Negative Strand Viruses pp 65–87 Edited by Mahy B. W. J., Barry R. D. New York London: Academic Press;
    [Google Scholar]
  19. Leppert M., Rittenhouse L., Perrault J., Summers D. F., Kolakofsky D. 1979; Plus and minus strand leader RNAs in negative strand virus-infected cells. Cell 18:735–747
    [Google Scholar]
  20. McGowan J. J., Emerson S. U., Wagner R. R. 1982; The plus-strand leader RNA of VSV inhibits DNA-dependent transcription of adenovirus and SV40 genes in a soluble whole cell extract. Cell 28:325–333
    [Google Scholar]
  21. Marx P. A., Portner A., Kingsbury D. W. 1974; Sendai virion transcriptase complex: polypeptide composition and inhibition by virion envelope proteins. Journal of Virology 13:107–112
    [Google Scholar]
  22. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  23. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 6:677–684
    [Google Scholar]
  24. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxovirus: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  25. Perrault J., Clinton G. M., McClure M. A. 1983; RNP template of vesicular stomatitis virus regulates transcription and replication functions. Cell 35:175–185
    [Google Scholar]
  26. Raghow R., Portner A., Hsu C. H., Clark S. B., Kingsbury D. W. 1978; Charge heterogeneity in polypeptides of negative strand RNA viruses. Virology 90:214–225
    [Google Scholar]
  27. Rinke J., Steitz J. A. 1982; Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies. Cell 29:149–159
    [Google Scholar]
  28. Rose J. K. 1978; Complete sequences of the ribosome recognition sites in vesicular stomatitis virus mRNAs: recognition by the 40S and 80S complexes. Cell 14:345–353
    [Google Scholar]
  29. Rose J., Iverson L. 1979; Nucleotide sequence from the 3′ end of vesicular stomatitis virus mRNAs as determined from cloned DNA. Journal of Virology 32:404–411
    [Google Scholar]
  30. Rose K., Kocher H. P., Blumberg B. M., Kolakofsky D. 1983a; An improved procedure, involving mass spectrometry, for Nα-terminal amino acid sequence determination of proteins which are Nα-blocked. Biochemical Journal 217:253–257
    [Google Scholar]
  31. Rose K., Simona M. G., Offord R. E. 1983b; Amino acid sequence determination by g.l.c.-mass spectrometry of permethylated peptides. Biochemical Journal 215:261–272
    [Google Scholar]
  32. Roux L., Kolakofsky D. 1974; Protein kinase associated with Sendai virions. Journal of Virology 13:545–547
    [Google Scholar]
  33. Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K. 1983; Sequence of 3, 687 nucleotides from the 3′ end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Research 11:7317–7330
    [Google Scholar]
  34. Sprague Condra J. H., Arnheiter H., Lazzarini R. A. 1983; Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein. Journal of Virology 45:773–781
    [Google Scholar]
  35. Stone H. O., Kingsbury D. W., Darlington R. W. 1972; Sendai virus-induced transcriptase from infected cells: polypeptides in the transcriptase complex. Journal of Virology 10:1037–1043
    [Google Scholar]
  36. Szilagyi J. F., Uryvaev L. 1973; Isolation of an infectious ribonucleoprotein from vesicular stomatitis virus containing an active RNA transcriptase. Journal of Virology 11:279–286
    [Google Scholar]
  37. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. 1969; Protein composition of the structural components of vesicular stomatitis virus. Journal of Virology 3:611–618
    [Google Scholar]
  38. Week P. K., Wagner R. R. 1979; VSV infection reduces the number of active DNA-dependent RNA polymerases in myeloma cells. Journal of Biological Chemistry 254:5430–5434
    [Google Scholar]
  39. Wilusz J., Kurilla M. G., Keene J. D. 1983; A host protein (La) binds to a unique species of minus-sense leader RNA during replication of vesicular stomatitis virus. Proceedings of the National Academy of Sciences, U. S. A 80:5827–5831
    [Google Scholar]
  40. Wold F. 1981; In vivo chemical modification of proteins. Annual Review of Biochemistry 50:783–814
    [Google Scholar]
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