1887

Abstract

Summary

Recently, methods have been developed to culture dissociated tracheal epithelial (TE) cells. Attempts were made to infect these epithelial cells with mouse cytomegalovirus (MCMV) to see if the dissociated epithelial cells share characteristics of infection with MCMV-infected tracheal organ cultures. When isolated TE cells were incubated with MCMV at multiplicities between 0.10 and 2.0, no infection or minimal infection resulted. Centrifugation of virus onto the TE cell sheets also resulted in only minimal infection. If MCMV-infected mouse embryo fibroblasts were added to cultures of TE cells, however, the TE cells subsequently became infected. TE cells could also be infected by incubating MCMV with mixed cultures of uninfected fibroblasts and TE cells. The epithelial nature of infected cells was confirmed by electron microscopy. Reconstruction experiments demonstrated that fibroblast-mediated infection of mouse TE cells with MCMV was not simply due to the large amounts of virus provided by the infected fibroblasts. It is suggested that cell-cell contact, or fusion with infected cells is required for productive infection of TE cells with MCMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-4-671
1984-04-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/4/JV0650040671.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-4-671&mimeType=html&fmt=ahah

References

  1. Benyesh-Melnick M. 1969; Cytomegaloviruses. In Diagnostic Procedures for Viral and Rickettsial Infections pp 701–732 Edited by Lennette E. H., Schmidt N. J. New York: American Public Health Association;
    [Google Scholar]
  2. Dutko F. J., Oldstone M. B. A. 1981; Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. Journal of Experimental Medicine 154:1636–1651
    [Google Scholar]
  3. Fong C. K. A., Bia F., Hsiung G. D. 1980; Ultrastructural development and persistence of guinea pig cytomegalovirus in duct cells of guinea pig submaxillary gland. Archives of Virology 64:97–108
    [Google Scholar]
  4. Gray T. E., Thomassen D. G., Mass M. J., Barrett J. C. 1983; Quantitation of cell proliferation, colony formation, and carcinogen induced cytotoxicity of rat tracheal epithelial cells grown in culture on 3T3 feeder layers. In Vitro 19:559–570
    [Google Scholar]
  5. Henson D., Neapolitan C. 1970; Pathogenesis of chronic mouse cytomegalovirus infection in submaxillary glands of C3H mice. American Journal of Pathology 58:255–267
    [Google Scholar]
  6. Hudson J. B., Misra V., Mosmann T. R. 1976; Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity. Virology 72:235–243
    [Google Scholar]
  7. Mäntyjärvi R. A., Selgrade M. J., Collier A. M., Hu S., Pagano J. S. 1977; Murine cytomegalovirus infection of epithelial cells in mouse tracheal ring organ culture. Journal of Infectious Diseases 136:444–448
    [Google Scholar]
  8. Mims C. A. 1974; Factors in the mechanism of persistence of viral infections. Progress in Medical Virology 18:1–14
    [Google Scholar]
  9. Muller M. T., Hudson J. B. 1977; Cell cycle dependency of murine CMV replication in synchronized 3T3 cells. Journal of Virology 22:267–272
    [Google Scholar]
  10. Nedrud J. G., Wu R. 1982; Growth of murine tracheal epithelial cells in hormone supplemented medium on collagen substrates. Federation Proceedings 41:622
    [Google Scholar]
  11. Nedrud J. G., Collier A. M., Pagano J. S. 1979; Cellular basis for susceptibility to mouse cytomegalovirus: evidence from tracheal organ culture. Journal of General Virology 45:737–744
    [Google Scholar]
  12. Nedrud J. G., Pagano J. S., Collier A. M. 1982; Long-term mouse cytomegalovirus infection of tracheal organ culture: relation to host cell replication. Journal of General Virology 60:247–259
    [Google Scholar]
  13. Osborn J. E., Walker D. L. 1968; Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation. Journal of Virology 2:853–858
    [Google Scholar]
  14. Reynolds D. W., Stagno S., Stubbs G., Dahle A. J., Livingston M. M., Saxon S. S., Alford C. A. 1974; Inapparent congenital cytomegalovirus infection with elevated cord IgM levels: causal relation with auditory and mental deficiency. New England Journal of Medicine 290:291–296
    [Google Scholar]
  15. Rheinwald J. G., Green H. 1975; Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–344
    [Google Scholar]
  16. Selgrade M. J. K., Nedrud J. G., Collier A. M., Gardner D. E. 1981; Effects of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infection and Immunity 33:840–847
    [Google Scholar]
  17. Wu R., Groelke J. W., Chang L. Y., Porter M. E., Smith D., Nettesheim P. 1982; Effects of hormones on the multiplication and differentiation of tracheal epithelial cells in culture. In Growth of Cells in Hormonally Defined Media Cold Spring Harbor Conferences on Cell Proliferation vol 9 pp 641–656 Edited by Sirbasku D. A., Sato G. H., Pardee A. B. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-4-671
Loading
/content/journal/jgv/10.1099/0022-1317-65-4-671
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error