1887

Abstract

SUMMARY

An RNA-dependent polymerase associated with rice gall dwarf virus (RGDV), a new Phytoreovirus, was characterized. Optimum polymerase activity was found between pH 8.0 and 8.5 at 25°C. Mg was essential for the activity, the optimum concentration being 2 m. Mn could replace Mg. The RNA products synthesized hybridized with RGDV RNA, but not with RNA of rice dwarf virus which is in the same subgroup of plant reoviruses. Electrophoretic analysis of the products showed at least nine bands, the sizes of which are discussed.

Keyword(s): dsRNA , RGDV and RNA polymerase
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/content/journal/jgv/10.1099/0022-1317-65-3-533
1984-03-01
2024-04-24
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References

  1. Black D. R., Knight C. A. 1970; Ribonucleic acid transcriptase activity in purified wound tumor virus. Journal of Virology 6:194–198
    [Google Scholar]
  2. Hibi T., Omura T., Saito V. 1981; Double-stranded RNA of rice gall dwarf virus. (Abstract) Annals of the Phytopathological Society of Japan 47:412 (in Japanese)
    [Google Scholar]
  3. Kodama T., Suzuki N. 1973; RNA polymerase activity in purified rice dwarf virus. Annals of the Phytopathological Society of Japan 39:251–258
    [Google Scholar]
  4. Lewandowski L. J., Kalmakoff J., Tanada Y. 1969; Characterization of a ribonucleic acid polymerase activity associated with purified cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. Journal of Virology 4:857–865
    [Google Scholar]
  5. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  6. McMaster G. K., Carmichael G. G. 1977; Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U,. S,. A 74:4835–4838
    [Google Scholar]
  7. Omura T., Morinaka T., Inoue H., Saito Y. 1982; Purification and some properties of rice gall dwarf virus, a new Phytoreovirus. Phytopathology 72:1246–1249
    [Google Scholar]
  8. Reddy D. V. R., Rhodes D. P., Lesnaw J. A., MacLeod R., Banerjee A. K., Black L. M. 1977; In vitro transcription of wound tumor virus RNA by virion-associated RNA transcriptase. Virology 80:356–361
    [Google Scholar]
  9. Shatkin A. J., Sipe J. D. 1968; RNA polymerase activity in purified reoviruses. Proceedings of the National Academy of Sciences, U,. S,. A 61:1462–1469
    [Google Scholar]
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