1887

Abstract

SUMMARY

An RNA-dependent polymerase associated with rice gall dwarf virus (RGDV), a new Phytoreovirus, was characterized. Optimum polymerase activity was found between pH 8.0 and 8.5 at 25°C. Mg was essential for the activity, the optimum concentration being 2 m. Mn could replace Mg. The RNA products synthesized hybridized with RGDV RNA, but not with RNA of rice dwarf virus which is in the same subgroup of plant reoviruses. Electrophoretic analysis of the products showed at least nine bands, the sizes of which are discussed.

Keyword(s): dsRNA , RGDV and RNA polymerase
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/content/journal/jgv/10.1099/0022-1317-65-3-533
1984-03-01
2022-01-20
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