1887

Abstract

SUMMARY

Hybridomas were developed that secreted antibodies to soybean mosaic (SMV), lettuce mosaic (LMV) viruses, and to maize dwarf mosaic (MDMV) virus, strains Ap and B. All hybridomas produced antibodies specific to the homologous virus except for one to LMV, which produced antibodies that reacted at a low level with SMV and MDMV. Monoclonal antibodies against SMV were used in a double-antibody sandwich radioimmunoassay. The assay using only one type of monoclonal antibody lacked sensitivity because, presumably, limited epitopes were available. Results obtained using two monoclonal antibodies that bound to different epitopes were comparable to those using polyclonal antibodies. A competitive radioimmunoassay, using a single monoclonal antibody, was developed to detect successfully SMV, LMV and MDMV.

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1984-03-01
2024-04-23
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References

  1. Al Moudallal Z., Briand J. P., van Regenmortel M. H. V. 1982; Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 1:1005–1010
    [Google Scholar]
  2. Ball E. M. 1974 Serological tests for the identification of plant viruses St. Paul: The American Phytopathological Society;31 pp
    [Google Scholar]
  3. Berger P. H. 1980; The retention of infectivity of maize dwarf mosaic virus in aphids. Implications for long-distance dispersal M.S. thesis University of Minnesota;
    [Google Scholar]
  4. Berger P. H., Toler R. W., Harris K. F. 1983; Maize dwarf mosaic virus transmission by greenbug biotypes. Plant Disease 67:496–497
    [Google Scholar]
  5. Briand J. P., Al Moudallal Z., van Regemortel M. H. V. 1982; Serological differentiation of tobamoviruses by means of monoclonal antibodies. Journal of Virological Methods 5:293–300
    [Google Scholar]
  6. Bryant G. R., Durand D. P., Hill J. H. 1983; Development of solid-phase radioimmunoassay for detection of soybean mosaic virus. Phytopathology 73:623–629
    [Google Scholar]
  7. Chen L. C., Durand D. P., Hill J. H. 1982; Detection of pathogenic strains of soybean mosaic virus by enzyme-linked immunosorbent assay with polystyrene plates and beads as the solid phase. Phytopathology 72:1177–1181
    [Google Scholar]
  8. Cho E. K., Goodman R. M. 1979; Strains of soybean mosaic virus: classification based on virulence in resistant soybean lines. Phytopathology 69:467–470
    [Google Scholar]
  9. Clark M. F. 1981; Immunosorbent assays in plant pathology. Annual Review of Phytopathology 19:83–106
    [Google Scholar]
  10. Diaco R., Lister R. M., Durand D. P., Hill J. H. 1983; Production of monoclonal antibodies against three isolates of barley yellow dwarf virus. Phytopathology 73:788
    [Google Scholar]
  11. Dietzgen R. G., Sander E. 1982; Monoclonal antibodies against a plant virus. Archives of Virology! 4:197–204
    [Google Scholar]
  12. Flamand A., Wiktor T. J., Koprowski H. 1980; Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein. Journal of General Virology 48:97–107
    [Google Scholar]
  13. Galfré G., Milstein C. 1981; Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73:3–46
    [Google Scholar]
  14. Galfré G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  15. Ghabrial S. A., Shepherd R. J. 1980; A sensitive radioimmunosorbent assay for the detection of plant viruses. Journal of General Virology 48:311–317
    [Google Scholar]
  16. Halk E. L., Hsu H. T., Aebig J. 1982a; Properties of virus specific monoclonal antibodies to prunus necrotic ringspot (NRSV), apple mosaic (ApMV), tobacco streak (TSV), and alfalfa mosaic (AMV) viruses. Phytopathology 72:953
    [Google Scholar]
  17. Halk E. L., Hsu H. T., Aebig J., Chang K. 1982b; Production of hybrid cell lines secreting virus specific monoclonal antibodies to three ilarviruses and alfalfa mosaic virus. Phytopathology 72:938
    [Google Scholar]
  18. Hill E. K., Durand D. P., Hill J. H. 1983; Production and use of monoclonal antibodies against the potato virus Y group. Annual Meeting of the American Society for Microbiology TI9 p 299
    [Google Scholar]
  19. Hill J. H., Benner H. I. 1980; Properties of soybean mosaic virus and its isolated protein. Phytopathologische Zeitschrift 97:272–281
    [Google Scholar]
  20. Hill J. H., Bryant G. R., Durand D. P. 1981; Detection of plant virus by using purified IgG in ELISA. Journal of Virological Methods 3:27–35
    [Google Scholar]
  21. Koprowski H., Gerhard W., Croce C. M. 1977; Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proceedings of the National Academy of Sciences, U,. S,. A 74:2985–2988
    [Google Scholar]
  22. Langenberg W. G. 1973; Serology, physical properties, and purification of unaggregated infectious maize dwarf mosaic virus. Phytopathology 63:149–154
    [Google Scholar]
  23. McLaughlin M. R., Bryant G. R., Hill J. H., Benner H. I., Durand D. P. 1980; Isolation of specific antibody to plant viruses by acid sucrose density gradient centrifugation. Phytopathology 73:831–834
    [Google Scholar]
  24. Purcifull D. E. 1966; Some properties of tobacco etch virus and its alkaline degradation products. Virology 29:8–14
    [Google Scholar]
  25. van Deusen R. A., Whetstone C. A. 1981; Practical aspects of producing and using anti-viral monoclonal antibodies as diagnostic reagents. Annual Proceedings of the American Association of Veterinary Laboratory Diagnosticians 24:211–228
    [Google Scholar]
  26. Voller A., Bidwell D. E., Bartlett A. 1979; The enzyme-labeled immunosorbent assay (ELISA). Alexandria: Dynatech Laboratories, 128 pp;
    [Google Scholar]
  27. Yelton D. E., Scharff M. D. 1981; Monoclonal antibodies: a powerful new tool in biology and medicine. Annual Review of Biochemistry 50:657–680
    [Google Scholar]
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