Different Sizes of Restriction Endonuclease Fragments from the Terminal Repetitions of the Herpes Simplex Virus Type 1 Genome Latent in Trigeminal Ganglia of Mice Free

Abstract

SUMMARY

Trigeminal ganglion DNA from mice latently infected with herpes simplex virus type 1 was analysed by restriction enzyme digestion, agarose gel electrophoresis and blot hybridization to P-labelled viral DNA. Viral DNA from parental virions and from virions obtained as a consequence of reactivation by ganglion neurectomy were similarly analysed. The HI restriction fragments of parental and reactivated virions were almost indistinguishable from each other, and several of the larger HI fragments of viral DNA were also found in latently infected ganglia at unaltered sizes. In contrast, fractionation of RI fragments of latently infected ganglion DNA by reverse phase column (RPC-5) chromatography, followed by gel electrophoresis and blot hybridization to a viral DNA probe from the S component terminal repetition, revealed the presence of several terminal fragments at discrete sizes ranging from 1 kb to 15 kb, quite unlike the 5.7 kb terminal RI K fragment of virion-derived DNA. These results indicate that structural changes occur in the viral genome concomitantly with the establishment of latency, such as may result from extensive gene rearrangement or integration into cellular DNA.

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1984-02-01
2024-03-28
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References

  1. Baringer J. R., Swoveland P. 1973; Recovery of herpes simplex virus from human trigeminal ganglions. New England Journal of Medicine 288:648–650
    [Google Scholar]
  2. Bastian F. O., Rabson A. S., Yee C. L., Tralka T. S. 1972; Herpesvirus hominis: isolation from human trigeminal ganglia. Science 178:306–307
    [Google Scholar]
  3. Brown S. M., Subak-sharpe J. H., Warren K. G., Wroblewska A., Koprowski H. 1979; Detection of complementation of defective or uninducible (herpes simplex type 1) virus genomes latent in human ganglia. Proceedings of the National Academy of Sciences, U.S.A. 76:2364–2368
    [Google Scholar]
  4. Cabrera C. V., Wohlenberg C., Openshaw H., Rey-mendez M., Puga A., Notkins A. L. 1980; Herpes simplex virus DNA sequences in the CNS of latently infected mice. Nature, London 288:288–290
    [Google Scholar]
  5. Denniston K. J., Madden M. J., Enquist L. W., vanDe woude G. 1981; Characterization of coliphage lambda hybrids carrying DNA fragments from herpes simplex type 1 defective interfering particles. Gene 15:365–378
    [Google Scholar]
  6. Fraser N. W., Lawrence W. C., Wroblewska Z., Gilden D. H., Koprowski H. 1981; Herpes simplex type 1 DNA in human brain tissue. Proceedings of the National Academy of Sciences, U. S. A. 78:6461–6465
    [Google Scholar]
  7. Hardies S. C., Wells R. D. 1976; Preparative fractionation of DNA restriction fragments by reversed phase column chromatography. Proceedings of the National Academy of Sciences, U.S.A 73:3117–3121
    [Google Scholar]
  8. Locker H., Frenkel N. 1979; BamHI, Kpnl and Sail restriction enzyme maps of the DNAs of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. Journal of Virology 32:429–441
    [Google Scholar]
  9. Peden K., Mounts P., Hayward G. S. 1982; Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high complexity probe. Cell 31:71–80
    [Google Scholar]
  10. Price R. W., Schmitz J. 1978; Reactivation of latent herpes simplex virus infection of the autonomic nervous system by postganglionic neurectomy. Infection and Immunity 19:523–532
    [Google Scholar]
  11. Price R. W., Katz B. J., Notkins A. L. 1975; Latent infections of the peripheral ANS with herpes simplex virus. Nature, London 257:686–688
    [Google Scholar]
  12. Puga A., Rosenthal J. D., Openshaw H., Notkins A. L. 1978; Herpes simplex virus DNA and mRNA sequences in acutely and chronically infected trigeminal ganglia of mice. Virology 89:102–111
    [Google Scholar]
  13. Puga A., Cantin E. M., Notkins A. L. 1982; Homology between murine and human cellular DNA sequences and the terminal repetition of the S component of the herpes simplex virus type 1 genome. Cell 31:81–87
    [Google Scholar]
  14. Reyes G. R., La Femina R., Hayward S. D., Hayward G. S. 1979; Morphological transformation by DNA fragments of human herpesviruses: evidence for two distinct transforming regions in herpes simplex virus types 1 and 2 and lack of correlation with biochemical transfer of the thymidine kinase gene. Cold Spring Harbor Symposia on Quantitative Biology 44:629–641
    [Google Scholar]
  15. Reyes G. R., Mclane M. W., Hayward G. S. 1982; Transfection with isolated herpes simplex virus thymidine kinase genes. II. Evidence for amplification of viral and adjacent cellular DNA sequences. Journal of General Virology 60:209–224
    [Google Scholar]
  16. Rock D. L., Fraser N. W. 1983; Detection of HSV-1 genome in central nervous system of latently infected mice. Nature, London 302:523–525
    [Google Scholar]
  17. Roizman B. 1979; The structure and isomerization of herpes simplex virus genomes. Cell 16:481–494
    [Google Scholar]
  18. Stevens J. G., Cook M. L. 1971; Latent herpes simplex virus in spinal ganglia of mice. Science 173:843–845
    [Google Scholar]
  19. Tilgham S. M., Tiemeier D. C., Polsky F., Edgell M. H., Seidman J. G., Leder A., Enquist L. W., Norman B., Leder P. 1977; Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences. Proceedings of the National Academy of Sciences, U. S. A. 74:4406–4410
    [Google Scholar]
  20. Wagner M. M., Summers W. C. 1978; Structure of the joint region and the termini of the DNA of herpes simplex virus type 1. Journal of Virology 27:374–387
    [Google Scholar]
  21. Walz M. A., Price W. A., Notkins A. L. 1974; Latent infection with herpes simplex virus type 1 and 2: viral reactivation in vivo after neurectomy. Science 184:1185–1187
    [Google Scholar]
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