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Volume 65, Issue 2

Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles Free

Abstract

SUMMARY

A polypeptide of 21500 mol. wt., structurally associated with hepatitis B virus core particles, was shown to have two kinds of HBeAg antigenicity (HBeAg/1 and HBeAg/2). This was revealed by transferring a single core peptide from polyacrylamide gels to nitrocellulose sheets (Western blotting), which reacted with anti-HBeAg/1 and anti-HBeAg/2. Selective discrimination of the two HBe antigens was achieved by radioimmunoassay (RIA). When highly purified core particles were incubated at 37°C in a 0.1% SDS-0.1% 2-mercaptoethanol solution, only HBeAg/1 was released after 5 min incubation and the release of HBeAg/2 occurred only after prolonging incubation for 30 min. The course of degradation was also detected by CsCl density gradient centrifugation. These results indicate that HBeAg/1 is less closely associated with core particles than is HBeAg/2. Electron microscopy showed that the core particles from which HBeAg/1 was removed were more labile than the original preparation when incubated at 56 °C in aqueous solution, or at 37 °C in Sarkosyl solutions; when placed in 1 -NaCl or -CsCl solution, the particles swelled to a larger diameter than untreated cores.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): association , EM , HBeAg subtypes and HBV core particles
© Journal of General Virology 1984
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1984-02-01
2024-04-19
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Immunological and Morphological Properties of HBeAg Subtypes (HBeAg/1 and HBeAg/2) in Hepatitis B Virus Core Particles
J. Gen. Virol. 65, 405 (1984); https://doi.org/10.1099/0022-1317-65-2-405
/content/journal/jgv/10.1099/0022-1317-65-2-405
/content/journal/jgv/10.1099/0022-1317-65-2-405
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