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Journal of General Virology header logo Journal of General Virology

Volume 65, Issue 2

Purification, Morphology and Antigenic Characterization of Measles Virus Envelope Components No Access

Abstract

SUMMARY

Measles virus haemagglutinin (H), fusion (F) and matrix (M) components were purified by affinity chromatography using monoclonal antibodies coupled to CNBr-activated Sepharose. H and M proteins were purified to homogeneity as determined by polyacrylamide gel electrophoresis by a single cycle of adsorption-desorption. The corresponding purification of the F protein required two cycles of adsorption-desorption. After the second adsorption an extra wash with 1 -guanidine-HCl was employed to remove the contaminating cellular actin. Electron microscopic examination of the purified envelope proteins showed that, at pH 6.0, the H peplomers had a truncated conical shape (width 6.5 to 4 nm, length 16 nm), and the F peplomers had a club-like shape (dimensions of the oval head 6 × 9 nm, length 15 nm). Lengths of both peplomers were measured excluding their undiscernible hydrophobic part. The M component at pH 3.0 appeared as a rounded particle (diam. 8 nm, central accumulation of contrast 1.5 nm) suggested to include four to six M polypeptides. Rabbit hyperimmune sera were prepared against all three purified envelope components. These sera reacted only with the homologous antigen in radioimmunoprecipitation assays. Both antisera against the H and F components neutralized the virus and blocked virus-specific haemolysis, but only anti-H serum inhibited haemagglutination.

  • Received:
  • Accepted:
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Keyword(s): envelope proteins , isolation , measles virus and morphology and antigenicity
© Journal of General Virology 1984
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1984-02-01
2025-01-14
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/content/journal/jgv/10.1099/0022-1317-65-2-355
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Purification, Morphology and Antigenic Characterization of Measles Virus Envelope Components
J. Gen. Virol. 65, 355 (1984); https://doi.org/10.1099/0022-1317-65-2-355
/content/journal/jgv/10.1099/0022-1317-65-2-355
/content/journal/jgv/10.1099/0022-1317-65-2-355
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