Several strains of the enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV) have been shown to replicate in alveolar macrophages maintained in vitro. A distinct cytopathic effect was observed at a multiplicity of infection ≥0.1. Infected cells released infectious virus. The extent of both virus production and cell destruction was highly dependent upon the virus input. At low input, cell viability was affected only slightly, and a delayed and persistent virus production could be observed. TGEV infection of macrophages also led to a marked synthesis of type I interferon. Thus, the possibility that alveolar macrophages act as an extra-intestinal target for TGEV must be considered.
BohlE. H.,
GuptaR. K. P.,
McCloskeyL. W.,
SaifL.1972; Immunology of transmissible gastroenteritis. Journal of the American Veterinary Medical Association 160:543–549
ChuR. M.,
GlockR. D.,
RossR. F.1982; Changes in gut associated lymphoid tissues of the small intestine of eight-week-old pigs infected with transmissible gastroenteritis virus. American Journal of Veterinary Research 43:67–76
FuruuchiS.,
ShimizuY.,
KumagaiT.1978/79; Multiplication of low and high cell culture passaged strains of transmissible gastroenteritis virus in organs of newborn piglets. Veterinary Microbiology 3:169–178
La BonnardièreC.,
LaudeH.1981; High interferon titer in newborn pig intestine during experimentally induced viral enteritis. Infection and Immunity 32:28–31
LaudeH.,
GelfiJ.,
AynaudJ. M.1981; In vitro properties of low- and high-passaged strains of transmissible gastroenteritis of swine. American Journal of Veterinary Research 42:447–449
PattersonS.,
MacnaughtonM. R.1982; Replication of human respiratory coronavirus strain 229E in human macrophages. Journal of General Virology 60:307–314
PedersenN. C.1976; Morphologic and physical characteristics of feline infectious peritonitis virus and its growth in autochthonous peritoneal cell cultures. American Journal of Veterinary Research 137:567–573
PensaertM.,
HaeltermanE. O.,
BurnsteinT.1970; Transmissible gastroenteritis of swine: virus-intestinal cell interactions. Archiv für die gesamte Virusforschung 31:321–334
UnderdahlN. R.,
MebusC. A.,
StairE. L.,
RhodesM. B.,
McGillL. D.,
TwiehausM. J.1974; Isolation of transmissible gastroenteritis virus from lungs of market-weight swine. American Journal of Veterinary Research 35:1209–1216
UnderdahlN. R.,
MebusC. A.,
Torres-MedinaA.1975; Recovery of transmissible gastroenteritis virus from chronically infected experimental pigs. American Journal of Veterinary Research 36:1473–1476
VirelizierJ. L.1981; Role of macrophages and interferon in natural resistance to mouse hepatitis virus infection. Current Topics in Microbiology and Immunology 92:51–64
WardleyR. C.,
HamiltonF.,
WilkinsonP. J.1979; The replication of virulent and attenuated strains of African swine fever virus in porcine macrophages. Archives of Virology 61:217–225