1887

Abstract

SUMMARY

The satellite RNA species from five isolates of tomato black ring virus (TBRV), belonging to two serotypes, and one isolate of myrobalan latent ringspot virus (MLRV) were compared. Each was translated in wheat germ extracts to give a single prominent polypeptide of mol. wt. 48000 (TBRV satellites) or 45000 (MLRV satellite). Analysis of the translation products by peptide mapping, and of the satellite RNA molecules by oligonucleotide mapping, showed that the MLRV satellite RNA was distinct from any of the others. The TBRV satellites had some similarities to each other but were of two types that differed in nucleotide sequence and that were associated with TBRV helper isolates of the different serotypes.

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1984-02-01
2022-08-08
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References

  1. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  2. Delbos R., Dunez J., Barrau J., Fisac R. 1976; The RNAs of three strains of tomato black ring virus. Annales de Microbiologie 127A:101–103
    [Google Scholar]
  3. De Wachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  4. Dunez J., Delbos R. 1976; Myrobalan latent ringspot, a bipartite genome virus and a strain of tomato black ring virus. Mitteilungen der Biologischen Bundesanstalt für Land-u. Forstwirtschaft 170:9–16
    [Google Scholar]
  5. Fritsch C., Mayo M. A., Murant A. F. 1978; Translation of the satellite RNA of tomato black ring virus in vitro and in tobacco protoplasts. Journal of General Virology 40:587–593
    [Google Scholar]
  6. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  7. Gallitelli D., Piazzolla P., Savino V., Quacquarelli A., Martelli G. P. 1981; A comparison of myrobalan latent ringspot virus with other nepoviruses. Journal of General Virology 53:57–65
    [Google Scholar]
  8. Harrison B. D. 1958; Relationship between beet ringspot, potato bouquet and tomato black ring viruses. Journal of General Microbiology 18:450–460
    [Google Scholar]
  9. Hollings M. 1965; Some properties of celery yellow vein, a virus serologically related to tomato black ring virus. Annals of Applied Biology 55:459–470
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Murant A. F., Mayo M. A. 1982; Satellites of plant viruses. Annual Review of Plant Pathology 20:49–70
    [Google Scholar]
  12. Murant A. F., Raschké J. H. 1981; Relationships among nepoviruses. Annual Report of the Scottish Horticultural Research Institute, 1980 pp 95–96
    [Google Scholar]
  13. Murant A. F., Raschké J. H. 1982; Specificity of the dependence of satellite RNA on tomato black ring virus (TBRV). Annual Report of the Scottish Crop Research Institute, 1981 pp 107–108
    [Google Scholar]
  14. Murant A. F., Taylor M. 1978; Estimates of molecular weights of nepovirus RNA species by polyacrylamide gel electrophoresis under denaturing conditions. Journal of General Virology 41:53–61
    [Google Scholar]
  15. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  16. Pedersen F. S., Haseltine W. A. 1980; A micromethod for detailed characterization of high molecular weight RNA. Methods in Enzymology 65:680–687
    [Google Scholar]
  17. Robinson D. J. 1982; Apparent lack of nucleotide sequence homology between the satellite and genome RNA species of tomato black ring virus. Journal of General Virology 58:453–456
    [Google Scholar]
  18. Taylor C. E., Murant A. F. 1969; Transmission of strains of raspberry ringspot and tomato black ring viruses by Longidorus elongatus (de Man). Annals of Applied Biology 64:43–48
    [Google Scholar]
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