@article{mbs:/content/journal/jgv/10.1099/0022-1317-65-12-2277, author = "Exley, T. and Parti, S. and Barwick, S. and Meager, A.", title = "A Comparison of the Neutralizing Properties of Monoclonal and Polyclonal Antibodies to Human Interferon Alpha", journal= "Journal of General Virology", year = "1984", volume = "65", number = "12", pages = "2277-2280", doi = "https://doi.org/10.1099/0022-1317-65-12-2277", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-12-2277", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "neutralization", keywords = "monoclonal and polyclonal antibodies", keywords = "IFN (HuIFN-α)", abstract = "SUMMARY Specific polyclonal antisera to human interferon-α1 (HuIFN-α1), human interferon-α2 (HuIFN-α2) and human lymphoblastoid interferon (HuIFN-αLy Namalwa) have been raised in rabbits and sheep. The antisera raised against HuIFN-α1 and HuIFN-α2 strongly neutralized the antiviral activity of their homologous IFN-α subtypes, but were less active against the heterologous IFN-α subtypes and preparations containing mixtures of IFN-α subtypes, e.g. human leukocyte interferon (HuIFN-αLe). Antisera raised against HuIFN-αLy Namalwa strongly neutralized the antiviral activity of all IFN-α-containing preparations and showed weak cross-reactivity with human interferon-β (HuIFN-β). Neither anti-HuIFN-α1 nor anti-HuIFN-α2 could be demonstrated to neutralize the antiviral activity of HuIFN-β. A number of monoclonal antibodies to HuIFN-α2 have been prepared and these were found to neutralize HuIFN-α2 antiviral activity to varying degrees, but not to neutralize the heterologous subtype HuIFN-α1, preparations containing mixtures of IFN-α subtypes or HuIFN-β.", }