1887

Abstract

SUMMARY

Specific polyclonal antisera to human interferon-1 (HuIFN-1), human interferon-2 (HuIFN-2) and human lymphoblastoid interferon (HuIFN-Ly Namalwa) have been raised in rabbits and sheep. The antisera raised against HuIFN-1 and HuIFN-2 strongly neutralized the antiviral activity of their homologous IFN- subtypes, but were less active against the heterologous IFN- subtypes and preparations containing mixtures of IFN- subtypes, e.g. human leukocyte interferon (HuIFN-Le). Antisera raised against HuIFN-Ly Namalwa strongly neutralized the antiviral activity of all IFN--containing preparations and showed weak cross-reactivity with human interferon- (HuIFN-). Neither anti-HuIFN-1 nor anti-HuIFN-2 could be demonstrated to neutralize the antiviral activity of HuIFN-. A number of monoclonal antibodies to HuIFN-2 have been prepared and these were found to neutralize HuIFN-2 antiviral activity to varying degrees, but not to neutralize the heterologous subtype HuIFN-1, preparations containing mixtures of IFN- subtypes or HuIFN-.

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/content/journal/jgv/10.1099/0022-1317-65-12-2277
1984-12-01
2022-11-30
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