1887

Abstract

SUMMARY

By electron microscopy, particles of defective interfering Semliki Forest virus (DI SFV) had a mean diameter of 46.8 nm compared with 55.9 nm for standard virus particles, a decrease of 16%. The difference was confirmed by measurements of the two-dimensional projected areas of DI and standard virus particles. We examined nine different DI virus preparations produced by four to 13 undiluted passages in BHK cells and all were found to contain a majority of the smaller type of particle. Calculation of the absolute number of small particles showed that there were 130 particles per interfering unit measured by the inhibition of virus RNA synthesis. However, a more sensitive assay based on interference with virus protein synthesis gave a particle:interference ratio of 6.5.

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1984-12-01
2024-04-18
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References

  1. Barrett A. D. T., Crouch C. F., Dimmock N. J. 1981; Assay of defective-interfering Semliki Forest virus by theinhibition of synthesis of virus-specified RNAs. Journal of General Virology 54:273–280
    [Google Scholar]
  2. Barrett A. D. T., Crouch C. F., Dimmock N. J. 1984; Defective interfering Semliki Forest virus populations arebiologically and physically heterogeneous. Journal of General Virology 65:1273–1283
    [Google Scholar]
  3. Brown D. T., Gliedman J. B. 1973; Morphological variants of Sindbis virus obtained from infected mosquitotissue culture cells. Journal of Virology 12:1534–1539
    [Google Scholar]
  4. Bruton C. J., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: structural differences between standard virus and defective-interfering particles. Journal of General Virology 31:383–395
    [Google Scholar]
  5. Bruton C. J., Porter A., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: intra-cellular events during interference. Journal of General Virology 31:397–416
    [Google Scholar]
  6. Eaton B. T. 1975; Defective interfering particles of Semliki Forest virus do not interfere with viral RNAsynthesis in Aedes albopictus cells. Virology 68:534–538
    [Google Scholar]
  7. Eaton B. T., Faulkner P. 1973; Altered pattern of viral RNA synthesis in cells infected with standard anddefective Sindbis virus. Virology 51:85–93
    [Google Scholar]
  8. Guild G. M., Stollar V. 1975; Defective interfering particles of Sindbis virus. III. Intracellular viral RNAspecies in chick embryo cell cultures. Virology 67:24–41
    [Google Scholar]
  9. Holland J. J., Kennedy S. I. T., Semler B. L., Jones C. L., Roux L., Grabau E. A. 1980; Defective interferingRNA viruses and the host cell response. In Comprehensive Virology vol 16: pp 137–192 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  10. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10: pp 73–146 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  11. Johnston R. E., Wan K., Bose H. R. 1974; Homologous interference induced by Sindbis virus. Journal ofVirology 14:1076–1082
    [Google Scholar]
  12. Johnston R. E., Tovell D. R., Brown D. T., Faulkner P. 1975; Interfering passages of Sindbis virus:concomitant appearance of interference, morphological variants and truncated viral RNA. Journal ofVirology 16:951–958
    [Google Scholar]
  13. Kääriäinen L., Pettersson R. F., Kerränen S., Lehtovaara P., Söderlund H., Ukkonen P. 1981; Multiplestructurally related defective interfering RNAs formed during undiluted passages of Semliki Forest virus. Virology 113:686–697
    [Google Scholar]
  14. Kowal K. J., Stollar V. 1980; Differential sensitivity of infectious and defective interfering particles ofSindbis virus to ultraviolet irradiation. Virology 103:149–157
    [Google Scholar]
  15. Laine R., Söderlund H., Renkonen O. 1973; Chemical composition of Semliki Forest virus. Intervirology 1:110–118
    [Google Scholar]
  16. Lehtovaara P., Söderlund H., Keränen S., Pettersson R. F., Kääriäinen L. 1981; 18S defective interferingRNA of Semliki Forest virus contains a triplicated linear repeat. Proceedings of the National Academy ofSciences, U.S.A 78:5353–5357
    [Google Scholar]
  17. Lehtovaara P., Söderlund H., Keränen S., Pettersson R. F., Kääriäinen L. 1982; Extreme ends of thegenome are conserved and rearranged in the defective interfering RNAs of Semliki Forest virus. Journal ofMolecular Biology 156:731–748
    [Google Scholar]
  18. Logan K. B. 1979; Generation of defective interfering particles of Semliki Forest virus in a clone of Aedesalbopictus (mosquito) cells. Journal of Virology 30:38–44
    [Google Scholar]
  19. Perrault J. 1981; Origin and replication of defective interfering particles. Current Topics in Microbiology andImmunology 93:151–207
    [Google Scholar]
  20. Schlesinger S., Schlesinger M., Burge B. W. 1972; Defective virus particles from Sindbis virus. Virology 48:615–617
    [Google Scholar]
  21. Shenk T. E., Stollar V. 1972; Viral RNA species in BHK-21 cells infected with Sindbis virus serially passagedat high multiplicity of infection. Biochemical and Biophysical Research Communications 49:60–67
    [Google Scholar]
  22. Shenk T. E., Stollar V. 1973; Defective interfering particles of Sindbis virus. I. Isolation and some chemicaland biological properties. Virology 53:162–173
    [Google Scholar]
  23. Stollar V. 1979; Defective interfering particles of togaviruses. Current Topics in Microbiology and Immunology 86:35–66
    [Google Scholar]
  24. Stollar V. 1980; Defective interfering alphaviruses. In The Togaviruses pp 427–457 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  25. Weiss B., Schlesinger S. 1973; Defective interfering passages of Sindbis virus: chemical composition, bio-logical activity and mode of interference. Journal of Virology 12:862–871
    [Google Scholar]
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