1887

Journal of General Virology header logo

Volume 65, Issue 12

Molecular Cloning of Rauscher Spleen Focus-forming Virus and Biological Properties of the Cloned Virus Free

Abstract

SUMMARY

Rauscher virus (RV) induces acute erythroleukaemia and a myeloproliferative disease in adult mice. It consists of a replication-competent murine leukaemia virus (R-MuLV) which acts as a helper virus and a defective transforming component which causes spleen focus formation, Rauscher spleen focus-forming virus (R-SFFV). The integrated proviral DNA of R-SFFV was cloned molecularly. The cloned R-SFFV was compared to that of other viral components which are associated with RV-induced disease and also to the cloned Friend SFFV (F-SFFV) and the myeloproliferative sarcoma virus (MPSV), both of which expand the erythroid (F-SFFV, MPSV) and myeloid (MPSV) compartment on infection of adult mice. The genome of R-SFFV differs, if analysed by restriction enzymes, from R-MuLV in the 3′ end of the genome between the gene and the long terminal repeat. The difference is most likely an alteration in the 3′ part of the gp70-coding region of the gene. Comparison with Rauscher mink cell focus-inducing virus (R-MCF) suggests that R-SFFV is derived from R-MCF by substitution of the 3′ half of the gene with a sequence of unknown origin. The molecularly cloned R-SFFV pseudotyped with Friend MuLV induces an increase in late erythroid precursor cells which still require erythropoietin for maturation. Host range studies of the molecularly cloned R-SFFV prove that the locus is required but not sufficient to restrict RV-induced haemopoiesis in adult mice, thus suggesting that R-SFFV has a different target cell range than F-SFFV and is similar to MPSV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biological properties , molecular cloning and R-SFFV
© Journal of General Virology 1984
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-12-2225
1984-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/12/JV0650122225.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-12-2225&mimeType=html&fmt=ahah

References

  1. Benton W. D., Davis R. W. 1977; Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180–182
     [Google Scholar]
  2. Bestwick R., Ruta M., Kiessling A., Faust C., Linemeyer D., Scolnick E., Kabat D. 1983; Genetic Structure of Rauscher spleen focus-forming virus. Journal of Virology 45:1217–1222
     [Google Scholar]
  3. Bilello J. A., Colletta G., Warnecke G., Koch G., Frisby D., Pragnell I. B., Ostertag W. 1980; Analysis of the expression of spleen focus-forming virus (SFFV)-related RNA and gp55, a Friend and Rauscher virus- specific protein. Virology 107:331–344
     [Google Scholar]
  4. Clark S. P., Mak T. W. 1984; Fluidity of a retroviral genome. Journal of Virology 50:759–765
     [Google Scholar]
  5. De Wet J. R., Daniels D. L., Schroeder J. L., Williams B. G., Denniston-Thompson K., Moore D. D., Blattner F. R. 1980; Restriction maps for twenty-one Charon vector phages. Journal of Virology 33:401–410
     [Google Scholar]
  6. Fagg B., Ostertag W. 1982; Friend erythroleukemia virus complex: role of viral components in modifying erythroid differentiation in mice. Journal of the National Cancer Institute 68:457–460
     [Google Scholar]
  7. Habara A., Reddy E. P., Aaronson S. A. 1982; Rauscher murine leukemia virus: molecular cloning of infectious integrated proviral DNA. Journal of Virology 44:731–735
     [Google Scholar]
  8. Hasthorpe S., Bol S. 1979; Erythropoietin responses and physical characterization of erythroid progenitor cells in Rauscher virus infected BALB/c mice. Journal of Cellular Physiology 100:77–86
     [Google Scholar]
  9. Kaminchick J., Hankins D. W., Ruscetti F. K., Linemeyer D. L., Scolnick E. M. 1982; Molecular cloning of biologically active proviral DNA of the anemia-inducing strain of spleen focus-forming virus. Journal of Virology 44:922–931
     [Google Scholar]
  10. Klein B., Le Bousse C., Fagg B., Smadja-Joffe F., Vehmeyer K., Mori K. J., Jasmin C., Ostertag W. 1981; Effects of myeloproliferative sarcoma virus on the pluripotential stem cell and granulocyte precursor cell populations of DBA/2 mice. Journal of the National Cancer Institute 66:935–940
     [Google Scholar]
  11. Koch W., Zimmermann W., Oliff A., Friedrich R. 1984; Molecular analysis of the envelope gene and long terminal repeat of Friend mink cell focus-inducing virus: implications for the functions of these sequences. Journal of Virology 49:828–840
     [Google Scholar]
  12. Kollek R., Stocking C., Smadja-Joffe F., Ostertag W. 1984; Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants. Journal of Virology 50:717–724
     [Google Scholar]
  13. Linemeyer D. L., Ruscetti S. K., Menke J. G., Scolnick E. M. 1980; Recovery of biologically active spleen focus-forming virus from molecularly cloned spleen focus-forming virus-pBR322 circular DNA by cotransfection with infectious type C retroviral DNA. Journal of Virology 35:710–721
     [Google Scholar]
  14. Linemeyer D. L., Menke J. G., Ruscetti S. K., Evans L. H., Scolnick E. M. 1982; Envelope gene sequences which encode the gp52 protein of spleen focus-forming virus are required for the induction of erythroid cell proliferation. Journal of Virology 43:223–233
     [Google Scholar]
  15. Machida C. A., Bestwick R. K., Kabat D. 1984; Reduced leukemogenicity caused by mutations in the membrane glycoprotein gene of Rauscher spleen focus-forming virus. Journal of Virology 49:394–402
     [Google Scholar]
  16. Mol J. N. M., Ostertag W., Bilello J., Stoof T. J. 1982; Rauscher spleen focus-forming virus: biological properties and relationship to helper viruses. Journal of General Virology 63:45–56
     [Google Scholar]
  17. Odaka T. 1973; Inheritance of susceptibility to Friend mouse leukemia virus. X. Separate genetic control of two viruses in Friend virus preparations. International Journal of Cancer 11:567–574
     [Google Scholar]
  18. Opitz U., Seidel H.-J., Rich I. S. 1977; Erythroid stem cells in Rauscher virus-infected mice. Blut 35:35–44
     [Google Scholar]
  19. Ostertag W., Pragnell I. B. 1981; Differentiation and viral involvement in differentiation of transformed mouse and rat erythroid cells. Current Topics in Microbiology and Immunology 94/95:143–208
     [Google Scholar]
  20. Ostertag W., Vehmeyer K., Fagg B., Pragnell I. B., Paetz W., Le Bousse M. C., Smadja-Joffe F., Klein B., Jasmin C., Eisen H. 1980; Myeloproliferative virus, a cloned murine sarcoma virus with spleen focusforming properties in adult mice. Journal of Virology 33:573–582
     [Google Scholar]
  21. Ostertag W., Odaka T., Smadja-Joffe F., Jasmin C. 1981; Inheritance of susceptibility to the myeloproliferative sarcoma virus: effect of the Fv-2 locus and evidence for a myeloproliferative sarcoma virus resistance locus. Journal of Virology 37:541–548
     [Google Scholar]
  22. Ostertag W., Pragnell I. B., Fusco A., Hughes D., Freshney M., Klein B., Jasmin C., Bilello W., Warnecke G., Vehmeyer K. 1982; Studies on the biology and genetics of murine erythroleukemia viruses. In Expression of Differentiated Functions in Cancer Cells pp 401–416 Edited by Revoltella R. P., Pontieri G. M., Basilico C., Rovera G., Gallo R. C., Subak-Sharpe J. H. New York: Raven Press;
     [Google Scholar]
  23. Pragnell I. B., Ostertag W., Paul J. 1977; The expression of viral and globin genes during differentiation of the Friend cell. Experimental Cell Research 108:269–278
     [Google Scholar]
  24. Racevskis J., Koch G. 1977; Viral protein synthesis in Friend leukemia cell lines. Journal of Virology 21:328–337
     [Google Scholar]
  25. Rimm D. L., Horness D., Kucera J., Blattner F. R. 1980; Construction of coliphage lambda Charon vectors with fiumlll cloning sites. Gene 12:301–309
     [Google Scholar]
  26. Ruscetti S. K., Linemeyer D., Field J., Troxler D., Scolnick E. M. 1979; Characterization of a protein found in cells infected with the spleen focus-forming virus that shares immunological cross-reaction with gp-70 found in mink ceil focus inducing virus particles. Journal of Virology 30:787–798
     [Google Scholar]
  27. Ruta M., Kabat D. 1980; Plasma membrane glycoproteins encoded by cloned Rauscher and Friend spleen focus-forming viruses. Journal of Virology 35:844–853
     [Google Scholar]
  28. Seidel H. J., Opitz U. 1978; Comparative studies on hemopoietic stem cell pools in mice after Friend (FV-P) or Rauscher virus infection. In In Vitro Aspects of Erythropoiesis pp 143–148 Edited by Murphy M. J. Jr New York: Springer-Verlag;
     [Google Scholar]
  29. Stacey A., Arbuthnott C., Kollek R., Coggins L., Ostertag W. 1984; Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis. Journal of Virology 50:725–732
     [Google Scholar]
  30. Tambourin P. E. 1979; Haemopoietic stem cells and murine viral leukaemogenesis. Symposia of the British Society for Cell Biology 2: pp 259–316 Cambridge: Cambridge University Press;
     [Google Scholar]
  31. Troxler D. H., Ruscetti S. K., Scolnick E. M. 1980; The molecular biology of Friend virus. Biochimica et biophysica acta 605:303–324
     [Google Scholar]
  32. Van Griensven L. J. L. D., Vogt M. 1980; Rauscher ‘mink cell focus-inducing’ (MCF) virus causes erythroleukemia in mice: its isolation and properties. Virology 101:376–388
     [Google Scholar]
  33. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
     [Google Scholar]
  34. Vogt M. 1979; Properties of‘mink cell focus-inducing’ (MCF) virus isolated from spontaneous lymphoma lines of BALB/c mice carrying Moloney leukemia virus as an endogenous virus. Virology 93:226–236
     [Google Scholar]
  35. Vogt M. 1982; Virus cloned from the Rauscher virus complex induces erythroblastosis and thymic lymphoma. Virology 118:225–228
     [Google Scholar]
  36. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences, U.S.A 76:3683–3687
     [Google Scholar]
  37. Wolff L., Scolnick E., Ruscetti S. 1983; Envelope gene of the Friend spleen focus-forming virus: deletion and insertion in the 3′ gp70/pl5E-encoding region have resulted in unique features in the primary structure of its protein product. Proceedings of the National Academy of Sciences, U.S.A 80:4718–4722
     [Google Scholar]
  38. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–745
     [Google Scholar]
  39. Yamamoto Y., Gamble C. L., Clark S. P., Joyner A., Shibuya T., Macdonald M. E., Mager D., Bernstein A., Mak T. W. 1981; Clonal analysis of early and late stages of erythroleukemia induced by molecular clones of integrated spleen focus-forming viruses. Proceedings of the National Academy of Sciences, U.S.A 78:6893–6897
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-12-2225
Loading
Molecular Cloning of Rauscher Spleen Focus-forming Virus and Biological Properties of the Cloned Virus
J. Gen. Virol. 65, 2225 (1984); https://doi.org/10.1099/0022-1317-65-12-2225
/content/journal/jgv/10.1099/0022-1317-65-12-2225
/content/journal/jgv/10.1099/0022-1317-65-12-2225
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error