1887

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Volume 65, Issue 12

Biochemical and Serological Studies of Pathogenesis-related Proteins of Species Free

Abstract

SUMMARY

Pathogenesis-related proteins (PR proteins), found in leaves of different spp. infected with tobacco mosaic virus (TMV) or treated with potassium salicylate, were compared biochemically and also serologically using an antibody to PR1a purified from TMV-infected leaves of cv. Samsun NN. The antibody preparation reacted with PR proteins, PR1a, PR1b and PR1c, from four cultivars of and with a PR protein, b, from TMV-infected leaves of , but not with a PR protein, PR2, from cv. Samsun NN. The isoelectric points in 9 -urea of PR1a and PR1b from cv. Samsun NN were pH 4.3 and 4.8, respectively. Partial proteolysis of PR1a and PR1b with Pronase E yielded peptides which, although similar in size to those from PR1b, differed from them serologically. When V8 protease was used, the peptides released from each protein differed both in size and serological reaction. The patterns of peptides released from b of by V8 protease and Pronase E were different from those of peptides from PR1a and PR1b from . The antibody preparation reacted strongly with one of four peptides released by Pronase E and with two of the three peptides released by V8 protease. These results indicate that PR1a, PR1b and PR1c from and b from contain some similar antigenic determinants and have similar structures, and confirm that PR1a and PR1b are very similar but not identical in their primary structures.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): immunological blotting , Nicotiana , peptide mapping and PR proteins
© Journal of General Virology 1984
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1984-12-01
2024-04-25
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Biochemical and Serological Studies of Pathogenesis-related Proteins of Nicotiana Species
J. Gen. Virol. 65, 2209 (1984); https://doi.org/10.1099/0022-1317-65-12-2209
/content/journal/jgv/10.1099/0022-1317-65-12-2209
/content/journal/jgv/10.1099/0022-1317-65-12-2209
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