1887

Abstract

SUMMARY

Variable amounts of cDNA were synthesized from RNA extracted from several flaviviruses, including the four prototype dengue (DEN) virus serotypes. The synthesis was carried out using an oligo(dT) primer, suggesting the presence of a short poly(A) region at or near the 3′ end of some flavivirus genomes. The DEN-1 and DEN-2 prototype strains produced the largest amount of cDNA and were therefore used to investigate further the relatedness of flavivirus genomes by cDNA-RNA hybridization. The flaviviruses studied are related to each other to some extent since the hybrids formed exhibited about 30% S nuclease resistance, but a closer relationship was detected between dengue viruses of serotype 1 and 4 and between dengue virus serotype 2 and Edge Hill virus. A monoclonal antibody to the envelope protein (V3) of dengue viruses reacted with Edge Hill virus, confirming the genetic relationship between the viruses.

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/content/journal/jgv/10.1099/0022-1317-65-12-2173
1984-12-01
2021-10-23
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References

  1. Brawner T. A., Lee J. C., Trent D. W. 1977; A comparison of Saint Louis encephalitis and Sindbis virus RNA. Archives of Virology 54:147–151
    [Google Scholar]
  2. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  3. Doherty R. L. 1974; Arthropod-borne viruses in Australia. Progress in Medical Virology 17:136–192
    [Google Scholar]
  4. Doherty R. L. 1977; Arthropod-borne viruses in Australia, 19731976. Australian Journal of Experimental Biology and Medical Science 55:103–130
    [Google Scholar]
  5. Doherty R. L., Carley J. G., Gorman B. M. 1964; Studies of arthropod-borne virus infections in Queensland. 4. Further serological investigations of antibodies to group B arboviruses in man and animals. Australian Journal of Experimental Biology and Medical Science 42:149–164
    [Google Scholar]
  6. Doherty R. L., Carley J. G., Kay B. H., Filippich C., Marks E. N., Frazier C. L. 1979; Isolation of virus strains from mosquitoes collected in Queensland, 1972–1976. Australian Journal of Experimental Biology and Medical Science 57:509–520
    [Google Scholar]
  7. Gentry M. K., Henchal E. A., McCown J. M., Brandt W. E. 1982; Identification of distinct antigenic determinants of dengue-2 virus by using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 31:548–555
    [Google Scholar]
  8. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  9. Gough N. M., Webb E. A., Cory S., Adams J. M. 1980; Molecular cloning of seven mouse immunoglobulin K chain messenger ribonucleic acids. Biochemistry 19:2702–2710
    [Google Scholar]
  10. Gould A. R., Symons R. H. 1977; Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4:3787–3802
    [Google Scholar]
  11. Henchal E. A., Gentry M. K., McCown J. M., Brandt W. E. 1982; Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. American Journal of Tropical Medicine and Hygiene 31:830–836
    [Google Scholar]
  12. Monath T. P., Kinney R. M., Schlesinger J. J., Brandriss M. W., Brès P. 1983; Ontogeny of yellow fever 17D vaccine: RNA oligonucleotide fingerprint and monoclonal antibody analyses of vaccines produced worldwide. Journal of General Virology 64:627–637
    [Google Scholar]
  13. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  14. Porterfield J. S. 1980; Antigenic characteristics and classification of Togaviridae. In The Togaviruses pp 13–46 Edited by Schlesinger R. W. New York, San Francisco & London: Academic Press;
    [Google Scholar]
  15. Russell P. K., Nisalak A. 1967; Dengue virus identification by the plaque reduction neutralization test. Journal of Immunology 99:291–296
    [Google Scholar]
  16. Russell P. K., Brandt W. E., Dalrymple J. M. 1980; Chemical and antigenic structure of flaviviruses. In The Togaviruses pp 503–530 Edited by Schlesinger R. W. New York, San Francisco & London: Academic Press;
    [Google Scholar]
  17. Schlesinger R. W. 1977; Dengue viruses. Virology Monographs 16 Vienna & New York: Springer-Verlag;
    [Google Scholar]
  18. Strauss J. H., Strauss E. G. 1977; Togaviruses. In The Molecular Biology of Animal Viruses vol 1: pp 111–166 Edited by Nayak D. New York: Marcel Dekker;
    [Google Scholar]
  19. Trent D. 1977; Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. Journal of Virology 22:608–618
    [Google Scholar]
  20. Trent D. W., Grant J. A., Vorndam A. V., Monath T. P. 1981; Genetic heterogeneity among Saint Louis encephalitis virus isolates of different geographic origin. Virology 114:319–332
    [Google Scholar]
  21. Trent D. W., Grant J. A., Rosen L., Monath T. P. 1983; Genetic variation among dengue 2 viruses of different geographic origin. Virology 128:271–286
    [Google Scholar]
  22. Vezza A. C., Rosen L., Repik P., Dalrymple J., Bishop D. H. L. 1980; Characterization of the viral RNA species of prototype dengue viruses. American Journal of Tropical Medicine and Hygiene 29:643–652
    [Google Scholar]
  23. Walker P. J., Garrett S. T., Gorman B. M., Burke D. S. 1982; Genetic analysis of strain variation in dengue type 2 viruses. In Viral Diseases in South East Asia and the Western Pacific pp 513–516 Edited by Mackenzie J. S. Sydney: Academic Press;
    [Google Scholar]
  24. Wengler G., Wengler G., Gross H. J. 1978; Studies on virus-specific nucleic acids synthesized in vertebrate and mosquito cells infected with flaviviruses. Virology 89:423–437
    [Google Scholar]
  25. Westaway E. G. 1980; Replication of flaviviruses. In The Togaviruses pp 531–581 Edited by Schlesinger R. W. New York, San Francisco & London: Academic Press;
    [Google Scholar]
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