1887

Abstract

SUMMARY

bacteriophage SP3 was shown to be flagellotropic. Mechanical deflagellation of host strain 168wt prevented phage adsorption but adsorption was normal when deflagellated 186wt was allowed sufficient time to regenerate flagella. Two host strains lacking flagella (130 and 19 ) did not adsorb SP3. Two host strains with paralysed flagella (38 and 13 ) adsorbed SP3 inefficiently. Revertants of and strains to the motile phenotype adsorbed SP3 normally. A temperature-sensitive host mutant, 46, with reduced numbers of flagella at 46 °C was incapable of efficient SP3 adsorption at this temperature. However, transfection of 46 by SP3 DNA occurred with virtually identical efficiency at either temperature. Adsorption of a non-flagellotropic phage, SP82, to all bacterial strains used was unaffected by absence of flagella. Isolated flagella adsorbed SP3 inefficiently. Spontaneously non-motile strains of 168wt had lost the ability to support growth of SP3 but SP82 grew normally. Revertants of these strains to the motile phenotype regained the ability to support SP3 growth. Strains derived from surviving bacteria in SP3 lysates of 168wt were non-motile.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-11-2073
1984-11-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/11/JV0650112073.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-11-2073&mimeType=html&fmt=ahah

References

  1. Eiserling F., Romig W. 1962; Studies of Bacillus subtilis bacteriophages. Structural characterization by electron microscopy. Journal of Ultrastruclure Research 6:540–546
    [Google Scholar]
  2. Frankel R. W., Joys T. M. 1966; Adsorption specificity of bacteriophage PBS1. Journal of Bacteriology 92:388–389
    [Google Scholar]
  3. Grant G. F., Simon M. I. 1969; Synthesis of bacterial flagella. II. PBS1. Transduction of flagella-specific markers in Bacillus subtilis. Journal of Bacteriology 99:116–124
    [Google Scholar]
  4. Joys T. M. 1965; Correlation between susceptibility to bacteriophage PBS1 and motility in Bacillus subtilis. Journal of Bacteriology 90:1575–1577
    [Google Scholar]
  5. Lotz W., Acker G., Schmitt R. 1977; Bacteriophage 7-7-1 adsorbs to the complex flagella of Rhizobium lupini H13-3. Journal of General Virology 34:9–17
    [Google Scholar]
  6. Lovett P. S. 1972; PBP1: a flagella specific bacteriophage mediating transduction in Bacillus pumilis. Virology 47:743–752
    [Google Scholar]
  7. Meynell E. W. 1961; A phage Chi which attacks motile bacteria. Journal of General Microbiology 25:253–290
    [Google Scholar]
  8. Raimondo L. M., Lundh N. P., Martinez R. J. 1968; Primary adsorption site of phage PBS1: the flagellum of Bacillus subtilis. Journal of Virology 2:256–264
    [Google Scholar]
  9. Schade S. Z., Adler J., Ris H. 1967; How bacteriophage Chi attacks motile bacteria. Journal of Virology 1:599–609
    [Google Scholar]
  10. Shimizu N., Miura K., Aoki H. 1970; Characterization of Bacillus subtilis bacteriophage. II. Isolation and morphology of phage Nf and properties of its DNA. Journal of Biochemistry 68:277–286
    [Google Scholar]
  11. Spizizen J. 1959; Genetic activity of deoxyribonucleic acid in the reconstitution of biosynthetic pathways. Federation Proceedings 18:957–965
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-65-11-2073
Loading
/content/journal/jgv/10.1099/0022-1317-65-11-2073
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error