1887

Abstract

SUMMARY

Protoplasts from a virus-free isolate, 3b1a conD/A3, of var. were inoculated with a purified preparation of a mixture of four viruses, A, B1, B2 and C, from isolate 3b1a in the presence of polyethylene glycol. Three out of 30 fungal colonies obtained by regeneration of inoculated protoplasts were found to be infected, two with virus B1 and one with virus B2. The concentrations of these viruses in the newly infected isolates were similar to their respective concentrations in isolate 3b1a and remained stable through three serial subcultures.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-11-2061
1984-11-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/11/JV0650112061.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-11-2061&mimeType=html&fmt=ahah

References

  1. Boissonnet-Menes M., Lecoq H. 1976; Transmission de virus par fusion de protoplasts chez Pyricularia oryzae. Physiologie Vegetale 14:251–257
    [Google Scholar]
  2. Bozarth R. F. 1975; The problem and importance of transmission of mycovirus using cell free extracts. Abstracts of the Third International Congress for Virology (Madrid, 1975) 148:
    [Google Scholar]
  3. Buck K. W. 1984; A new double-stranded RNA virus from Gaeumannomyces graminis. Journal of General Virology 65:987–990
    [Google Scholar]
  4. Buck K. W., Almond M. R., McFadden J. J. P., Romanos M. A., Rawlinson C. J. 1981; Properties of thirteen viruses and virus variants obtained from eight isolates of the wheat take-all fungus, Gaeumannomyces graminis vartritici.. Journal of General Virology 53:235–245
    [Google Scholar]
  5. Cassells A. C., Barlass M. 1978; The initiation of tobacco mosaic virus infection in isolated protoplasts by polyethylene glycol. Virology 87:459–462
    [Google Scholar]
  6. Frick L. J., Lister R. M. 1978; Serotype variability in virus-like particles from Gaeumannomyces graminis. Virology 85:504–517
    [Google Scholar]
  7. Ghabrial S. A., Mernaugh R. L. 1983; Biology and transmission of Helminthosporium victoriae mycoviruses. In Double-stranded RNA Viruses pp 441–449 Edited by Compans R. W., Bishop D. H. L. Amsterdam, London & New York: Elsevier;
    [Google Scholar]
  8. Hamlyn P. F., Bradshaw R. E., Mellon F. M., Santiago C. M., Wilson J. M., Peberdy J. F. 1981; Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology 3:321–325
    [Google Scholar]
  9. Lai H. C., Zachariah K. 1975; Detection of virus-like particles in coremia of Penicillium claviforme. Canadian Journal of Genetics and Cytology 17:525–533
    [Google Scholar]
  10. Lapierre H., Lamaire J. M., Jouan B., Molin G. 1970; Mise en evidence des particules virales associées à une perte de pathogenicité chez le piétin-échaudage des cereales, Ophiobolus graminis Sacc. Comptes rendus hebdomadaires des seances de I’Académie des sciences, serie D 271:1833–1836
    [Google Scholar]
  11. Lemaire J. M., Lapierre H., Jouan B., Bertrand G. 1970; Découverte de particules virales chez certaines souches d’Ophiobolus graminis, agent du piétin-échaudage des céréales: consequences agronomiques prévisibles. Comptes rendus hebdomadaires des seances de I’Académie d’agriculture de France 56:1134–1137
    [Google Scholar]
  12. Lhoas P. 1971; Infection of protoplasts from Penicillium stoloniferum with double-stranded RNA viruses. Journal of General Virology 13:365–367
    [Google Scholar]
  13. Mcfadden J. J. P., Buck K. W., Rawlinson C. J. 1983; Infrequent transmission of double-stranded RNA virus particles but absence of DNA proviruses in single ascospore cultures of Gaeumarmomyces graminis. Journal of General Virology 64:927–937
    [Google Scholar]
  14. Maule A. J., Boulton M. L., Edmunds C., Wood K. R. 1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  15. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  16. Pallett I. H. 1976; Interactions between fungi and their viruses. In Microbial and Plant Protoplasts pp 107–124 Edited by Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. London: Academic Press;
    [Google Scholar]
  17. Rawlinson C. J., Buck K. W. 1981; Viruses in Gaeumannomyces and Phialophora spp. In Biology and Control of Take-all pp 151–172 Edited by Asher M. J. C., Shipton P. J. London & New York: Academic Press;
    [Google Scholar]
  18. Rawlinson C. J., Muthyalu G. 1974; Attempts to transmit fungal VLP. Report of the Rothamsted Experimental Station for 1973: part 1 119
    [Google Scholar]
  19. Rawlinson C. J., Hornby D., Pearson V., Carpenter J. M. 1973; Virus-like particles in the take-all fungus, Gaeumannomyces graminis. Annals of Applied Biology 74:197–209
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-11-2061
Loading
/content/journal/jgv/10.1099/0022-1317-65-11-2061
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error