Cell Division Does Not Affect Sendai Virus Genome Replication in Persistently Infected BHK Cells Free

Abstract

SUMMARY

The extent of Sendai virus genome replication in persistently infected BHK cells actively growing or at confluence was followed by estimation of the [H]uridine incorporated into intracellular nucleocapsid RNA. First, we showed that, in the presence of actinomycin D, actively growing persistently infected cells were taking up threefold more [H]uridine than resting cells. This higher uptake exhibited by growing cells was observed neither in persistently infected cells in the absence of actinomycin D, nor in acutely infected cells in the presence of actinomycin D. Assuming that the cellular pool of unlabelled uridine stays constant, we used a correction factor for this difference in [H]uridine uptake and estimated [H]uridine incorporation in nucleocapsid RNA, normalizing the data either to the amount of cell or of viral template. Results showed that the viral genome replication, expressed either way, was not significantly influenced by cell growth conditions.

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1984-11-01
2024-03-29
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References

  1. Ehrnst A. 1979; Growth phase related loss of measles virus surface-associated antigens and cytotoxic susceptibility in persistently infected cells. Journal of General Virology 45:547–556
    [Google Scholar]
  2. Holland J. J., Semler B. L., Jones C., Perrault J., Reid L., Roux L. 1978; Role of DI virus mutation and host response in persistent infections by enveloped RNA viruses. In Persistent Viruses, ICN-UCLA Symposia on Molecular and Cellular Biology vol XI: pp 57–73 Edited by Stevens J., Todaro G., Fox C. F. New York, San Francisco & London: Academic Press;
    [Google Scholar]
  3. Holland J. J., Kennedy S. I. T., Semler B. L., Jones C. L., Roux L., Grabau E. 1980; Defective interfering RNA viruses and the host cell response. In Comprehensive Virology vol 16: pp 137–192 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  4. Joseph B. S., Lampert P. W., Oldstone M. B. A. 1975; Replication and persistence of measles virus in defined subpopulations of human leukocytes. Journal of Virology 16:1638–1649
    [Google Scholar]
  5. Kolakofsky D. 1976; Isolation and characterization of Sendai virus DI RNAs. Cell 8:547–555
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Meddis R. 1975; Statistical Handbook for Non-Statisticians. Maidenhead: McGraw-Hill;
    [Google Scholar]
  9. Roux L., Holland J. J. 1979; Role of defective interfering particles of Sendai virus in persistent infections. Virology 93:91–103
    [Google Scholar]
  10. Roux L., Holland J. J. 1980; Viral genome synthesis in BHK 21 cells persistently infected with Sendai virus. Virology 100:53–64
    [Google Scholar]
  11. Roux L., Waldvogel F. A. 1981; Establishment of Sendai virus persistent infection: biochemical analysis of the early phase of a standard plus defective interfering virus infection of BHK cells. Virology 112:400–410
    [Google Scholar]
  12. Roux L., Waldvogel F. A. 1982; Instability of viral M protein in BHK 21 cells persistently infected with Sendai virus. Cell 28:293–302
    [Google Scholar]
  13. Roux L., Waldvogel F. A. 1983; Defective interfering particles of Sendai virus modulate HN expression at the surface of infected BHK cells. Virology 130:91–104
    [Google Scholar]
  14. Walker D. L. 1968; Persistent viral infection in cell culture. In Medical and Applied Virology pp 99–110 Edited by Sanders E. D., Lennette E. H. St Louis: Warren H. Green;
    [Google Scholar]
  15. Youngner J. S., Preble O. T. 1980; Viral persistence: evolution of viral populations. In Comprehensive Virology vol 16: pp 73–125 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
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