Interactions between Herpes Simplex Virus DNA-binding Proteins Free

Abstract

SUMMARY

Monoclonal antibodies directed against several herpes simplex virus (HSV)-induced DNA-binding proteins were used to investigate protein interactions in HSV-infected cells. Q1 monoclonal antibody, which is specific for the HSV-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major DNA-binding protein were bound. Conversely, when a monoclonal antibody to the major DNA-binding protein was used in affinity chromatography other polypeptides (including the DNA polymerase and alkaline nuclease) were eluted in addition to the major DNA-binding protein. Similar results were obtained when the experiment was performed using a monoclonal antibody to another HSV-2 DNA-binding protein. These results suggest the possibility that these polypeptides interact as part of the HSV DNA replication complex, and this hypothesis is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-11-2033
1984-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/11/JV0650112033.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-11-2033&mimeType=html&fmt=ahah

References

  1. Alberts B. M., Barry J., Bedinger P., Formosa T., Jongeneel C. V., Kreuzer K. N. 1982; Studies On DNA replication in the bacteriophage T4 in vitro system. Cold Spring Harbor Symposia on Quantitative Biology 47:655–688
    [Google Scholar]
  2. Banks L., Purifoy D. J. M., Hurst P-F., Killington R. A., Powell K. L. 1983; Herpes simplex virus nonstructural proteins. IV. Purification of the virus-induced deoxyribonuclease and characterization of the enzyme using monoclonal antibodies. Journal of General Virology 64:2249–2260
    [Google Scholar]
  3. Banks L. M., Vaughan P. J., Meredith D., Powell K. L. 1984; Monoclonal antibodies to herpes simplex virus thymidine kinase. Journal of General Virology 65:1625–1630
    [Google Scholar]
  4. Bayliss G. J., Marsden H. S., Hay I. 1975; Herpes simplex virus proteins: DNA binding proteins in infected cells and in the virus structure. Virology 68:124–134
    [Google Scholar]
  5. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  6. Darby G., Field H., Salisbury S. A. 1981; Altered substrate specificity of herpes simplex virus thymidine kinase confers acyclovir resistance. Nature, London 289:81–83
    [Google Scholar]
  7. Davison A. J., Wilkie N. M. 1983; Location and orientation of homologous sequences in the genomes of five herpesviruses. Journal of General Virology 64:1927–1942
    [Google Scholar]
  8. Evans A. S. 1982 Viral Infections of Humans 2nd edn New York: Plenum Press;
    [Google Scholar]
  9. Keir H. M., Gold E. 1963; Deoxyribonucleic acid nucleotidyltransferase and deoxyribonuclease from cultured cells infected with herpes simplex virus. Biochimica et biophysica acta 72:263–276
    [Google Scholar]
  10. Lee C. K., Knipe D. M. 1983; Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1. Journal of Virology 46:909–919
    [Google Scholar]
  11. Leinbach S. S., Casto J. F. 1983; Identification and characterisation of deoxyribonucleoprotein complexes containing the major DNA-binding protein of herpes simplex virus type 1. Virology 131:274–286
    [Google Scholar]
  12. Littler E., Yeo J., Killington R. A., Purifoy D. J. M., Powell K. L. 1981; Antigenic and structural conservation of herpesvirus DNA-binding proteins. Journal of General Virology 56:409–419
    [Google Scholar]
  13. Littler E., Purifoy D., Minson A., Powell K. L. 1983; Herpes simplex virus non-structural proteins. III. Function of the major DNA-binding protein. Journal of General Virology 64:983–995
    [Google Scholar]
  14. Pignatti P. F., Cassai E. 1980; Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells. Journal of Virology 36:816–828
    [Google Scholar]
  15. Pignatti P. F., Cassai E., Bertazzoni U. 1979; Herpes simplex virus DNA synthesis in a partially purified soluble extract from infected cells. Journal of Virology 32:1033–1036
    [Google Scholar]
  16. Powell K. L., Courtney R. J. 1975; Polypeptides synthesized in herpes simplex virus type 2-infected HEp-2 cells. Virology 66:217–228
    [Google Scholar]
  17. Powell K. L., Purifoy D. I. M. 1977; Nonstructural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase. Journal of Virology 24:618–626
    [Google Scholar]
  18. Powell K. L., Littler E., Purifoy D. I. M. 1981; Nonstructural proteins of herpes simplex virus. II. Major virus specific DNA binding protein. Journal of Virology 39:894–902
    [Google Scholar]
  19. Purifoy D. J. M., Benyesh-Melnick M. 1975; DNA polymerase induction by DNA-negative temperature-sensitive mutants of herpes simplex virus type 2. Virology 68:374–386
    [Google Scholar]
  20. Purifoy D. J. M., Powell K. L. 1976; DNA binding proteins induced by herpes simplex virus type 2 in HEp-2 cells. Journal of Virology 19:717–731
    [Google Scholar]
  21. Rawls W. E., Laurel D., Melnick J. L., Glicksman J. M., Kaufman R. H. 1968; A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of herpesviruses with distinct antigenic properties. American Journal of Epidemiology 87:647–655
    [Google Scholar]
  22. Sheldrick P., Berthelot N. 1975; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U. S. A 76:4350–4354
    [Google Scholar]
  24. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
  25. Yeo J., Killington R. A., Watson D. H., Powell K. L. 1981; Studies on cross reactive antigens in the herpesviruses. Virology 108:256–266
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-11-2033
Loading
/content/journal/jgv/10.1099/0022-1317-65-11-2033
Loading

Data & Media loading...

Most cited Most Cited RSS feed