Kilham Rat Virus DNA Replication in Subcellular Fractions Free

Abstract

SUMMARY

A subcellular system actively replicating Kilham rat virus DNA was developed. Cellular lysates and isolated nuclei from infected cells showed an amplification of replicative forms . Solubilized replicative complexes, either partly purified or in the form of a crude extract, were able to synthesize replicative forms and single-stranded DNA. DNA polymerase α played a major role in Kilham rat virus DNA synthesis and . Furthermore, a factor present in the cytosol of infected cells increased the polymerizing activity of viral replicative complexes.

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1984-11-01
2024-03-29
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