@article{mbs:/content/journal/jgv/10.1099/0022-1317-65-11-2011, author = "Wiegers, Frans P. and Vlak, Just M.", title = "Physical Map of the DNA of a Mamestra brassicae Nuclear Polyhedrosis Virus Variant Isolated from Spodoptera exigua", journal= "Journal of General Virology", year = "1984", volume = "65", number = "11", pages = "2011-2019", doi = "https://doi.org/10.1099/0022-1317-65-11-2011", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-65-11-2011", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "DNA map", keywords = "MNPV", keywords = "Mamestra brassicae MNPV", abstract = "SUMMARY The DNA of a multiply-enveloped nuclear polyhedrosis virus (MNPV) of the cabbage moth Mamestra brassicae (Mb), isolated from the beet armyworm Spodoptera exigua, was analysed using restriction endonucleases SmaI, BamHI, BglII and PstI. The size of the viral DNA was calculated to be about 152 kilobase pairs. Mapping of restriction sites on the DNA was accomplished by hybridization of 32P-labelled DNA fragments to single and double digests of the MbMNPV genome. Mapping of the putative polyhedrin gene was used to orient the physical map. The relevance of this map to the structural organization of the DNA of MbMNPV isolated from different geographical areas is discussed.", }