1887

Abstract

SUMMARY

The DNA molecule of varicella-zoster virus (VZV) is represented structurally as L-S, where L is a unique sequence and S is a unique sequence flanked by an inverted repeat. S may be present in either orientation in virion DNA molecules, but, to date, L has been found in only one orientation. DNA sequences were determined at the L-S joint and genome termini, which were cloned using methods designed to conserve either the 3′ or 5′ terminal nucleotide. Molecular hybridization experiments and analysis of the sequences showed that: (i) the genome is not terminally redundant; (ii) the unique sequence in L is flanked by an inverted repeat of 88.5 base pairs; (iii) a single unpaired nucleotide is located at each 3′ terminus of the genome, such that fusion of the termini would produce a sequence identical to that at the L-S joint; (iv) approx. 5% of virions contain genomes with L inverted. The implications of these results in possible mechanisms for VZV DNA replication are discussed.

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1984-11-01
2024-12-07
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References

  1. Ben-porat T., Rixon F. J. 1979; Replication of herpesvirus DNA. IV. Analysis of concatemers. Virology 94:61–70
    [Google Scholar]
  2. Ben-Porat T., Veach R. A. 1980; Origin of replication of the DNA of a herpesvirus (pseudorabies). Proceedings of the National Academy of Sciences, U. S. A 77:172–175
    [Google Scholar]
  3. Ben-Porat T., Rixon F. J., Blankenship M. L. 1979; Analysis of the structure of the genome of pseudorabies virus. Virology 95:285–294
    [Google Scholar]
  4. Davison A. J. 1983; DNA sequence of the Us component of the varicella-zoster virus genome. EMBO Journal 2:2203–2209
    [Google Scholar]
  5. Davison A. J., Rixon F. J. 1984; Cloning of the DNA of alphaherpesvirinae. In Developments in Molecular Virology vol 5 Edited by Becker Y. The Hague: Martinus Nijhoff; (in press)
    [Google Scholar]
  6. Davison A. J., Scott J. E. 1983; Molecular cloning of the varicella-zoster virus genome and derivation of six restriction endonuclease maps. Journal of General Virology 64:1811–1814
    [Google Scholar]
  7. Davison A. J., Wilkie N. M. 1981; Nucleotide sequence of the joint between the L and S segments of herpes simplex virus types 1 and 2. Journal of General Virology 55:315–331
    [Google Scholar]
  8. Davison A. J., Wilkie N. M. 1983a; Inversion of the two segments of the herpes simplex virus genome in intertypic recombinants. Journal of General Virology 64:1–18
    [Google Scholar]
  9. Davison A. J., Wilkie N. M. 1983b; Location and orientation of homologous sequences in the genomes of five herpesviruses. Journal of General Virology 64:1927–1942
    [Google Scholar]
  10. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  11. Dumas A. M., Geelen J. L. M. C., Maris W., Van Der Noordaa J. 1980; Infectivity and molecular weight of varicella-zoster virus DNA. Journal of General Virology 47:233–235
    [Google Scholar]
  12. Dumas A. M., Geelen J. L. M. C., Weststrate M. W., Wertheim P., Van Der Noordaa J. 1981; Xha I, Pst I and Bgl II restriction endonuclease maps of the two orientations of the varicella-zoster virus genome. Journal of Virology 39:390–400
    [Google Scholar]
  13. Ecker J. R., Hyman R. W. 1982; Varicella-zoster virus DNA exists as two isomers. Proceedings of the National Academy of Sciences, U,. S,. A 79:156–160
    [Google Scholar]
  14. Ecker J. R., Kudler L., Hyman R. W. 1984; Variation in the structure of varicella-zoster virus DNA. Inter virology 21:25–37
    [Google Scholar]
  15. Garoff H., Ansorge W. 1981; Improvements of DNA sequencing gels. Analytical Biochemistry 115:450–457
    [Google Scholar]
  16. Gilden D. H., Shtram Y., Friedmann A., Wellish M., Devlin M., Fraser N., Becker Y. 1982; The internal organization of the varicella-zoster virus genome. Journal of General Virology 60:371–374
    [Google Scholar]
  17. Jacob R. J., Morse L. S., Roizman B. 1979; Anatomy of herpes simplex virus DNA. XII. Accumulation of head-to-tail concatemers in nuclei of infected cells and their role in the generation of the four isomeric arrangements of viral DNA. Journal of Virology 29:448–457
    [Google Scholar]
  18. Ladin B. F., Blankenship M. L., Ben-Porat T. 1980; Replication of herpes virus DNA. V. Maturation of concatemeric DNA of pseudorabies virus to genome length is related to capsid formation. Journal of Virology 33:1151–1164
    [Google Scholar]
  19. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either strand of double-digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  20. Mocarski E. S., Roizman B. 1982a; Structure and role of the herpes simplex virus DNA termini in inversion, circularization and generation of virion DNA. Cell 31:89–97
    [Google Scholar]
  21. Mocarski E. S., Roizman B. 1982b; Herpesvirus-dependent amplification and inversion of cell-associated viral thymidine kinase gene flanked by viral a sequences and linked to an origin of viral DNA replication. Proceedings of the National Academy of Sciences, U,. S,. A 79:5626–5630
    [Google Scholar]
  22. Powell D. J. 1979 Structure and expression of the pseudorabies virus genome Ph.D thesis University of Glasgow;
    [Google Scholar]
  23. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 74:5463–5467
    [Google Scholar]
  25. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  26. Sheldrick P., Berthelot N. 1974; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  27. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  29. Straus S. E., Owens J., Ruyechan W. T., Tariff H. E., Casey T. A., Vande Woude G. F., Hay J. 1982; Molecular cloning and physical mapping of varicella-zoster virus DNA. Proceedings of the National Academy of Sciences. U. S. A 79:993–997
    [Google Scholar]
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