Equilibrium Density Gradient Centrifugation of the Scrapie Agent in Nycodenz

References

1. Bolton D. C., McKinley M. P., Prusiner S. B. 1982; Identification of a protein that purifies with the scrapie prion. Science 218:1309–1310
   [Google Scholar]
2. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
   [Google Scholar]
3. Diringer H., Geldeblom H., Hilmert H., Ozel M., Edelbluth C., Kimberlin R. H. 1983; Scrapie infectivity, fibrils and low molecular weight proteins. Nature, London 306:476–478
   [Google Scholar]
4. Dougherty R. M. 1964; Quantitation of viruses. In Techniques in Experimental Virology p 183 Edited by Harris R. J. C. New York: Academic Press;
   [Google Scholar]
5. Helenius A., Simons K. 1975; Solubilization of membranes by detergents. Biochimica et biophysica acta 415:29–79
   [Google Scholar]
6. Hjelmeland L. M., Chrambach A. 1984; Solubilization of functional membrane-bound receptors. In Membranes, Detergents and Receptor Solubilization Receptor Biochemistry and Methodology vol 1: pp 35–46 Edited by Venter J. C., Harrison L. C. New York: Alan R. Liss;
   [Google Scholar]
7. Kimberlin R. H., Marsh R. F. 1975; Comparison of scrapie and transmissible mink encephalopathy in hamsters. I. Biochemical studies of brain during development of disease. Journal of Infectious Diseases 131:97–103
   [Google Scholar]
8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
   [Google Scholar]
9. Lax A. J., Millson G. C., Manning E. J. 1983; Can scrapie titres be calculated accurately from incubation periods?. Journal of General Virology 64:971–973
   [Google Scholar]
10. Marsh R. F., Hanson R. P. 1978; The Syrian hamster as a model for the study of slow virus diseases caused by unconventional agents. Federation Proceedings 37:2076–2078
    [Google Scholar]
11. Marsh R. F., Dees C., Castle B. E., Wade W. F., German T. L. 1984; Purification of the scrapie agent by density gradient centrifugation. Journal of General Virology 65:415–421
    [Google Scholar]
12. Merz P. A., Somerville R. A., Wisniewski H. M., Iqbal K. 1981; Abnormal fibrils from scrapie-infected brain. Acta neuropathologica 54:63–74
    [Google Scholar]
13. Millson G. C., Hunter G. D., Kimberlin R. H. 1971; An experimental examination of the scrapie agent in cell membrane mixtures. II. Association of scrapie activity with membrane fractions. Journal of Comparative Pathology 81:255–265
    [Google Scholar]
14. Prusiner S. B. 1982; Novel proteinaceous infectious particles cause scrapie. Science 216:136–144
    [Google Scholar]
15. Prusiner S. B., Cochran S. P., Groth D. F., Downey D. E., Bowman K. A., Martinez H. M. 1981; Measurement of the scrapie agent using incubation time interval assay. Annals of Neurology 11:353–358
    [Google Scholar]
16. Semancik J. S., Marsh R. F., Geelen J. L. M. C., Hanson R. P. 1976; Properties of the scrapie agent-endomembrane complex from hamster brain. Journal of Virology 18:693–700
    [Google Scholar]
17. Sheetz M. P. 1979; Integral membrane protein interaction with Triton cytoskeletons of erythrocytes. Biochimica et biophysica acta 557:122–134
    [Google Scholar]
18. Vanden Berghe D. A. 1983; Comparison of various density-gradient media for isolation and characterization of animal viruses. In Iodinated Density-Gradient Media: A Practical Approach pp 175–192 Edited by Rickwood D. Oxford: IRE, Press;
    [Google Scholar]
19. Wray W., Boulikas T., Wray V. P., Hancock R. 1981; Silver staining of proteins in polyacrylamide gels. Analytical Biochemistry 118:197–203
    [Google Scholar]