1887

Abstract

SUMMARY

A systematic study has been performed using a series of differentiated rat thyroid epithelial cell lines either uninfected or infected with Kirsten murine sarcoma virus (KiMSV), to determine the levels of the p21 product of the oncogene in transformed and normal cell lines. The p21 levels have been assayed by SDS-polyacrylamide gel electrophoresis of immunoprecipitates of S-labelled cell extracts and by a GDP binding assay. All cell lines analysed showed a significant increase in the levels of p21 after transformation with KiMSV compared to the p21 levels of uninfected and untransformed differentiated thyroid cells. The results reported here confirm the potential ability of the oncogene product to transform epithelial cells. They show, furthermore, that not only is p21 present in some epithelial cells transformed by KiMSV, but also that it is functionally active, as has been shown for fibroblasts transformed by the same virus, and that its functioning is maintained after passaging of the transformed cells.

Keyword(s): epithelial , KiMSV , p21 and thyroid
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1984-11-01
2023-01-28
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References

  1. Ambesi-Impiombato F. S., Coon H. G. 1979; Thyroid cells in cultures. International Review of Cytology, Supplement 10:163–171
    [Google Scholar]
  2. Ambesi-Impiombato F. S., Parks L. A. M., Coon H. G. 1980; Culture of hormone-dependent functional epithelial cells from rat thyroid. Proceedings of the National Academy of Sciences, U. S. A 77:3455–3459
    [Google Scholar]
  3. Bilello J. A., Colletta G., Warnecke G., Koch G., Frisby D., Pragnell I. B., Ostertag W. 1980; Analysis of the expression of spleen focus-forming virus (SFFV)-related RNA and gp55, a Friend and Rauscher virus-specific protein. Virology 107:331–334
    [Google Scholar]
  4. Chang E. H., Furth M. E., Scolnick E. M., Lowy D. R. 1982; Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of the Harvey murine sarcoma virus. Nature, London 297:479–483
    [Google Scholar]
  5. Colletta G., Pinto A., Di Fiore P. P., Fusco A., Ferrentino M., Avvedimento V. E., Tsuchida N., Vecchio G. 1983; Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus. Molecular and Cellular Biology 3:2099–2109
    [Google Scholar]
  6. Der C. J., Krontiris T. G., Cooper G. M. 1982; Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras gene of Harvey and Kirsten sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 79:3637–3640
    [Google Scholar]
  7. Dhar R., Ellis W. R., Shih T. Y., Oroszlan S., Shapiro B., Maizel J., Lowy D., Scolnick E. 1982; Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus. Science 217:934–936
    [Google Scholar]
  8. Ellis R. W., Defeo D., Shih T. Y., Gonda M. A., Young H. A., Tsuchida N., Lowy D. R., Scolnick E. M. 1981; The p21 src genes of Harvey and Kirsten sarcoma virus originate from divergent members of a family of normal vertebrate genes. Nature, London 292:506–510
    [Google Scholar]
  9. Ellis R. W., Lowy D. R., Scolnick E. M. 1982; The viral and cellular p21 (ras) gene family. In Advances in Viral Oncology vol 1: pp 107–126 Edited by Klein G. New York: Raven Press;
    [Google Scholar]
  10. Furth M. E., Davis L. J., Fleurdelys G., Scolnick E. M. 1982; Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. Journal of Virology 43:294–304
    [Google Scholar]
  11. Fusco A., Pinto A., Ambesi-Impiombato F. S., Vecchio G., Tsuchida N. 1981; Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus. International Journal of Cancer 28:655–662
    [Google Scholar]
  12. Fusco A., Pinto A., Tramontano D., Tajana G., Vecchio G., Tsuchida N. 1982; Block in the expression of differentiation markers of rat thyroid epithelial cells by transformation with Kirsten murine sarcoma virus. Cancer Research 42:618–626
    [Google Scholar]
  13. Langbeheim H., Shih T. Y., Scolnick E. M. 1980; Identification of a normal vertebrate cell protein related to the p21 src of Harvey murine sarcoma virus. Virology 106:292–300
    [Google Scholar]
  14. Pulciani S., Santos E., Lauver A. V., Long L. K., Aaronson S. A., Barbacid M. 1982; Oncogenes in solid tumours. Nature, London 300:539–542
    [Google Scholar]
  15. Roy-Burman P., Klement V. 1975; Derivation of mouse sarcoma virus (Kirsten) by acquisition of genes from heterologous host. Journal of General Virology 28:193–198
    [Google Scholar]
  16. Scolnick E. M., Papageorge A. G., Shih T. Y. 1979; Guanine nucleoside-binding activity as an assay for src protein of rat-derived murine sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 76:5355–5359
    [Google Scholar]
  17. Shih T. Y., Weeks M. O., Young H. A., Scolnick E. M. 1979a; Identification of a sarcoma virus-coded phospho-protein in non-producer cells transformed by Kirsten or Harvey murine sarcoma virus. Virology 96:64–79
    [Google Scholar]
  18. Shih T. Y., Weeks M. O., Young H. A., Scolnick E. M. 1979b; p21 of Kirsten murine sarcoma virus is thermolabile in a viral mutant temperature sensitive for the maintenance of transformation. Journal of Virology 31:546–556
    [Google Scholar]
  19. Slamon D. J., Dekernion J. B., Verma L. M., Cline M. J. 1984; Expression of cellular oncogenes in human malignancies. Science 224:256–262
    [Google Scholar]
  20. Tabin C. I., Bradley S. M., Bargmann C. I., Weinberg R. A. 1982; Mechanism of activation of a human oncogene. Nature, London 300:143–149
    [Google Scholar]
  21. Tsuchida N., Ryder T., Ohtsubo E. 1982; Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus. Science 217:937–938
    [Google Scholar]
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