1887

Abstract

SUMMARY

Eighteen strains of Getah virus isolated from mosquitoes, swine and horses in Japan (1956 to 1981), and one strain isolated in Malaysia (1955), were analysed by RNase T1-resistant oligonucleotide fingerprinting. All fingerprints showed a poly(A) tract. The fingerprint pattern of the Malaysian strain was quite different from those of the Japanese strains. Although most of the recent Japanese isolates shared many large oligonucleotide spots in common, the patterns were not identical even among the strains obtained in one locality in the same year. These results suggest that the Getah virus genome undergoes mutation rather frequently. However, there is a tendency for the isolates of the same year to show greater similarity. The fingerprint patterns of certain host-dependent temperature-sensitive () mutants differed from that of the parental strain. Also, there were some differences in large oligonucleotide spots between strain JaNAr12380M isolated in suckling mouse brain (SMB) and strain JaNAr12380A isolated in C6/36 cells, despite the fact that both strains were derived from the same wild mosquito homogenate. In addition, many host-dependent mutants were present in strain JaNAr12380A, whereas no such mutants were observed in strain JaNAr12380M. It is concluded that there is considerable variation in the strains of Getah virus infecting mosquitoes in the wild, and also that the variants or mutants present in mosquitoes might be subject to selection during viral multiplication in the mammalian host.

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1984-11-01
2024-03-29
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References

  1. De Wachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  2. Elisberg B. L., Buescher E. L. 1963; Arbovirus catalogue card No. 47. Arbovirus Information Exchange
    [Google Scholar]
  3. Igarashi A. 1978; Isolation of a Singh’s Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. Journal of General Virology 40:531–544
    [Google Scholar]
  4. Igarashi A., Morita K., Bundo K., Matsuo S., Hayashi K., Matsuo R., Harada T., Tamoto H., Kuwatsuka M. 1981a; Isolation of Japanese encephalitis and Getah virus from Culex tritaeniorhynchus and slaughtered swine blood using Aedes albopictus clone C6/36 cells in Nagasaki, 1981. Tropical Medicine 23:177–187
    [Google Scholar]
  5. Igarashi A., Buei K., Ueba N., Yoshida M., Ito S., Nakamura H., Sasao F., Fukai K. 1981b; Isolation of viruses from female Culex tritaeniorhynchus in Aedes albopictus cell cultures. American Journal of Tropical Medicine and Hygiene 30:449–460
    [Google Scholar]
  6. Igarashi A., Makino Y., Matsuo S., Bundo K., Matsuo R., Higashi F., Tamoto Y., Kuwatsuka M. 1981c; Isolation of Japanese encephalitis and Getah viruses by Aedes albopictus clone C6/36 and by suckling mouse brain inoculation in Nagasaki, 1980. Tropical Medicine 23:69–78
    [Google Scholar]
  7. Igarashi A., Sasao F., Fukai K., Buei K., Ueba N., Yoshida M. 1981d; Mutants of Getah and Japanese encephalitis viruses isolates from field-caught Culex tritaeniorhynchus using Aedes albopictus clone C6/36 cells. Annals of Virology 132E:235–245
    [Google Scholar]
  8. Igarashi A., Matsuo S., Hayashi K., Oda T., Mori A., Fujita K., Itoh T. 1982; Isolation of Japanese encephalitis and Getah viruses from mosquitoes collected in Nagasaki city in the year of 1980 and 1981 using Aedes albopictus clone C6/36 cells. Tropical Medicine 24:47–53
    [Google Scholar]
  9. Kamada M., Kumanomido T., Ando Y., Fukunaga Y., Imagawa H., Wada R., Akiyama Y. 1982; Studies on Getah virus: some biological, physicoochemical and antigenic properties of the Ml-110 strain. Japanese Journal of Veterinary Science 44:89–96
    [Google Scholar]
  10. Kimura T., Ueba N. 1978; Some biological and serological properties of large and small plaque variants of Getah virus. Archives of Virology 57:221–229
    [Google Scholar]
  11. Marchette N. J., Rudnick A., Garcia R., Macvean D. W. 1978; Alphaviruses in peninsular Malaysia. 1. Virus isolations and animal serology. Southeast Asian Journal of Tropical Medicine and Public Health 9:317–329
    [Google Scholar]
  12. Marchette N. J., Rudnick A., Garcia R. 1980; Alphavirus in peninsular Malaysia. 2. Serological evidence of human infection. Southeast Asian Journal of Tropical Medicine and Public Health 11:14–23
    [Google Scholar]
  13. Matsuyama T., Nakamura T., Isahai K., Oya A., Kobayashi M. 1968; Haruna virus, a group A arbovirus isolated from swine in Japan. Virus 18:11–14
    [Google Scholar]
  14. Nakajima S., Nakajima K., Takeuchi Y., Sugiura A. 1980; Influenza surveillance based on oligonucleotide mapping of RNA of H1N1 viruses prevalent in Japan, 1978–1979. Journal of Infectious Diseases 142:492–502
    [Google Scholar]
  15. Nottay B. K., Kew O. M., Hatch M. H., Heyward J. T., Obijeski J. F. 1981; Molecular variation of type 1 vaccine-related and wild polioviruses during replication in humans. Virology 108:405–423
    [Google Scholar]
  16. Scherer W. F., Funkenbusch M., Buescher E. L., Izumi T. 1962; Sagiyama virus, a new group A arthropod-borne virus from Japan. American Journal of Tropical Medicine and Hygiene 11:255–268
    [Google Scholar]
  17. Sentsui H., Kono Y. 1980; An epidemic of Getah virus infection among racehorses: isolation of the virus. Research in Veterinary Science 29:157–161
    [Google Scholar]
  18. Sentsui H., Kono Y. 1981; Pathogenicity of Getah virus for mice. National Institute of Animal Health Quarterly 21:7–13
    [Google Scholar]
  19. Simpson D. I. H., Way H. J., Platt G. S., Bowen E. T. W., Hill M. N., Kamath S., Bendell P. J. E., Heathcote O. H. U. 1975; Arbovirus infections in Sarawak, October 1968-February 1970: Getah virus isolations from mosquitoes. Transactions of the Royal Society of Tropical Medicine and Hygiene 69:35–38
    [Google Scholar]
  20. Takigami M., Ishikawa H. 1978; Improved Cerenkov’s method of radioactivity measurement. Radioisotopes 27:123–127
    [Google Scholar]
  21. Tesh R. B., Gajdusek D. C., Garruto R. M., Cross J. H., Rosen L. 1975; The distribution and prevalence of group A arbovirus neutralizing antibodies among human populations in Southeast Asia and the Pacific islands. American Journal of Tropical Medicine and Hygiene 24:664–675
    [Google Scholar]
  22. Trent D. W., Grant J. A., Vorndam A. V., Monath T. P. 1981; Genetic heterogeneity among Saint Louis encephalitis virus isolates of different geographic origin. Virology 114:319–332
    [Google Scholar]
  23. Wada R., Kamada M., Fukunaga Y., Ando Y., Kumanomido T., Imagawa H., Akiyama Y. 1982; Equine Getah virus infection: pathological study of horses experimentally infected with the M1-110 strain. Japanese Journal of Veterinary Science 44:411–418
    [Google Scholar]
  24. Wengler G., Wengler G., Filipe A. R. 1977; A study of nucleotide sequence homology between the nucleic acids of different alphaviruses. Virology 78:124–134
    [Google Scholar]
  25. Yamamoto H. 1980; Arbovirus infections in the mosquitoes of Fukuoka area, Kyushu, Japan. 2. Natural infection of mosquitoes with the viruses of Getah complex in the period from 1963 to 1972. Japanese Journal of Sanitary Zoology 31:23–30
    [Google Scholar]
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