1887

Abstract

SUMMARY

Human B-cell lines, designated ATLB cell lines, were spontaneously established from peripheral blood of adult T-cell leukaemia (ATL) patients. The cell lines consistently expressed ATL-associated antigen (ATLA) and Epstein—Barr virus-associated nuclear antigen (EBNA). A cloned ATLB line, designated ATLB 2, showed that both ATLA and EBNA antigens were present in the same B-cell clone. In this study, we have further characterized ATLV and EBV in the cloned ATLB 2 cell line by biochemical techniques. The ATLA antigens in these clones, initially shown by immunofluorescence, were studied by immunoprecipitation with human sera from ATL patients and the Western blotting technique using a mouse monoclonal antibody (GIN-14). We identified ATLV core polypeptides 24K and 19K in the ATLB cell extracts. Furthermore, total cellular DNA and poly(A) RNA from the ATLB cell lines were analysed for the presence of viral genomes with molecularly cloned DNA probes containing the ATLV and EBV sequence, respectively. The results showed that all ATLB 2 clones contained highly conserved ATLV proviral DNA irrespective of whether or not they expressed ATLA. They also contained several copies of EB virus DNA and DNA—DNA reassociation kinetics studies clearly showed that most of the EBV DNA sequences were present in ATLB cells. ATLV-related mRNA was detected in only ATLA-positive clones (ATLB 2-3 and 2-21) but not in a negative clone (ATLB 2-45).

Keyword(s): B-cells , EB virus and human retrovirus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-10-1781
1984-10-01
2022-07-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/10/JV0650101781.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-10-1781&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Bornkamm G. W., Delius H., Zimber U., Hudewentz J., Epstein M. A. 1980; Comparison of Epstein-Barr virus strains of different origin by analysis of the viral DNAs. Journal of Virology 35:603–618
    [Google Scholar]
  3. Britten R. J., Davidson E. H. 1976; Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation. Proceedings of the National Academy of Sciences, U.S.A. 73:415–419
    [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutler W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  5. Deinhardt F., Deinhardt J. 1979; Comparative aspects: oncogenic animal herpesviruses. In The Epstein-Barr Virus pp 373–400 Edited by Epstein M. A., Achong B. G. Berlin: Springer-Yerlag;
    [Google Scholar]
  6. Gallo R. C., Mann D., Broder S., Ruscetti F. W., Maeda M., Kalyanaraman V. S., Robert-Guorff M., Reitz M. S. 1982; Human T-cell leukemia-lymphoma virus (HTLV) is in T but not B lymphocytes from a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences, U.S.A. 79:5680–5683
    [Google Scholar]
  7. Given D., Kieff E. 1978; DNA of Epstein-Barr virus. IV. Linkage map of restriction enzyme fragments of the B95-8 and W91 strains of Epstein-Barr virus. Journal of Virology 28:524–542
    [Google Scholar]
  8. Heller M., Danbaught T., Kieff E. 1981; Epstein-Barr virus DNA, IX. Variation among viral DNAs from producer and nonproducer infected cells. Journal of Virology 38:632–648
    [Google Scholar]
  9. Henle W., Henle G. 1979; Seroepidemiology of the virus. In The Epstein-Barr Virus pp 61–73 Edited by Epstein M. A., Achong B. G. Berlin: Springer-Verlag;
    [Google Scholar]
  10. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K., Shirakawa S., Miyoshi I. 1981; Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proceedings of the National Academy of Sciences, U.S.A 78:6476–6480
    [Google Scholar]
  11. Hinuma Y., Komoda H., Chosa T., Kondo T., Kohakura M., Takenaka T., Kikuchi M., Ichimaru M., Yunoki K., Sato L., Matsuo R., Takiuchi Y., Uchino H., Hanaoka M. 1982; Antibodies to adult T-cell leukemia virus-associated antigen (ATLA) in sera from patients with ATL and controls in Japan: a nation-wide sero-epidemiologic study. International Journal of Cancer 29:631–635
    [Google Scholar]
  12. Hirai K., Ikuta K., Kitamoto N., Kato S. 1981; Latency of herpesvirus of Turkey and Marek’s disease virus genomes in a chicken T-lymphoblastoid cell line. Journal of General Virology 53:133–143
    [Google Scholar]
  13. Kalyanaraman V. S., Sarngadharan M. G., Poiesz B. J., Ruscetti F. W., Gallo R. C. 1981; Immunological properties of a type-C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retrovirus. Journal of Virology 38:906–915
    [Google Scholar]
  14. Kalyanaraman V. S., Sarngadharan M. G., Nakao Y., Ito Y., Aoki T., Gallo R. C. 1982; Natural antibodies to the structure core protein (p24) of the human T-cell leukemia (lymphoma) retrovirus found in sera of leukemia patients in Japan. Proceedings of the National Academy of Sciences, U.S.A. 79:1653–1657
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. Miyoshi I., Kubonishi I., Sumida M., Hiraki S., Tsubota T., Kimura I., Miyamoto K., Sato J. 1980; A novel T-cell line derived from adult T-cell leukemia. Gann 71:155–156
    [Google Scholar]
  17. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. 1981; Detection of type-C virus particles in a cord T-cell line derived by cocultivation of normal human cord leukocytes and human leukemic T-cells. Nature, London 296:770–771
    [Google Scholar]
  18. Okada M., Koyanagi Y., Kobayashi N., Tanaka Y., Nakai M., Sano K., Takeuchi K., Hinuma Y., Hatanaka M., Yamamoto N. 1984; In vitro infection of human B lymphocytes with adult T-cell leukemia virus. Cancer Letters 22:11–21
    [Google Scholar]
  19. Poiesz B. J., Rucetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proceedings of the National Academy of Sciences, U.S.A 77:7415–7419
    [Google Scholar]
  20. Poiesz B. J., Ruscetti F. W., Reitz M. S., Kalyanaraman V. S., Gallo R. C. 1981; Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukemia. Nature, London 294:268–271
    [Google Scholar]
  21. Posner L. E., Robert-Guroff M., Kalyanaraman V., Poiesz B. J., Ruscetti F. W., Fossieck B., Bunn P. A. Jr, Minna J. D., Gallo R. C. 1981; Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas. Journal of Experimental Medicine 154:333–346
    [Google Scholar]
  22. Raab-Traub N., Pritchett R., Kieff E. 1978; DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus. Journal of Virology 27:388–398
    [Google Scholar]
  23. Robert-Guroff M., Nakao Y., Notake K., Ito Y., Sliski A., Gallo R. C. 1982; Natural antibodies to the human retrovirus HTLV in a cluster of Japanese patients with adult T-cell leukemia. Science 215:975–978
    [Google Scholar]
  24. Sabaran J. L., Hsu T. W., Yater C., Kaji A., Mason W. S., Taylor J. M. 1979; Analysis of integrated avian RNA tumor virus DNA in transformed chicken, duck and quail fibroblasts. Journal of Virology 29:170–178
    [Google Scholar]
  25. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  26. Srivastava B. I., Minowada J. 1973; Terminal deoxynucleotidyl transferase activity in a cell line (Molt-4) derived from the peripheral blood of a patient with acute lymphoblastic leukemia. Biochemical and Biophysical Research Communications 51:129–135
    [Google Scholar]
  27. Takatsuki K., Uchiyama T., Sagawa K., Yodoi J. 1977; Adult T-cell leukemia in Japan. In Topics in Hematology pp 73–77 Edited by Seno S., Takaku F., Irino S. Amsterdam: Excerpta Medica;
    [Google Scholar]
  28. Tanaka Y., Koyanagi Y., Chosa T., Yamamoto N., Hinuma Y. 1983; Monoclonal antibody reactive with both p28 and p 19 of adult T-cell leukemia virus specific polypeptides. Gann 74:327–330
    [Google Scholar]
  29. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  30. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A. 76:4250–4254
    [Google Scholar]
  31. Uchiyama T., Yodoi J., Sagawa K., Takatsuki K., Uchino H. 1977; Adult T-cell leukemia: clinical features and hematologic features of 16 cases. Blood 50:481–492
    [Google Scholar]
  32. Yamamoto N., Hinuma Y. 1982; Antigen in adult T-cell leukemia virus-producer cell line: reactivity with human serum antibodies. International Journal of Cancer 30:289–293
    [Google Scholar]
  33. Yamamoto N., Matsumoto T., Koyanagi Y., Tanaka Y., Hinuma Y. 1982; Unique cell lines harbouring both Epstein-Barr virus and adult T-cell leukemia (ATL) virus, established from ATL patients. Nature, London 299:367–369
    [Google Scholar]
  34. Yamamoto N., Schneider L., Koyanagi Y., Hinuma Y., Hunsmann G. 1983; Adult T-cell leukemia (ATL) virus specific antibodies in ATL patients and healthy virus carriers. International Journal of Cancer 32:281–287
    [Google Scholar]
  35. Yoshida M., Miyoshi I., Hinuma Y. 1982; Isolation and characterization of human adult T-cell leukemia and its implication in the disease. Proceedings of the National Academy of Sciences. U.S.A 29:2031–2035
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-10-1781
Loading
/content/journal/jgv/10.1099/0022-1317-65-10-1781
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error