1887

Abstract

SUMMARY

Antigenic and structural variation in the major nucleocapsid protein, VPN41, from different strains of respiratory syncytial (RS) virus was observed using a combination of monoclonal antibodies and two-dimensional peptide mapping. Limited trypsin treatment of intact nucleocapsids produced two peptide fragments 27K and 14K. Two monoclonal antibodies, N1 and N2, reactive with primary sequence epitopes located on intact nucleocapsids also reacted with either the 27K fragment (N2) or the 14K fragment (N1). Competitive radioimmunoassay studies using N1 and N2 antibodies revealed two discrete antigenic groups among the seven human strains of RS virus examined. A bovine strain of RS virus, although antigenically similar to the human strain of RS virus, was placed in a separate group. Two-dimensional peptide mapping of I-labelled VPN41 purified by SDS-PAGE revealed extensive structural homology between all strains. However, several unique tryptic/chymotryptic peptides supported the grouping obtained with the monoclonal antibodies.

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1984-10-01
2024-04-19
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References

  1. Bishop D. H. L., Roy P., Bean W. J., Simpson R. W. 1971; Transcription of the influenza ribonucleic acid genome by a virion polymerase. III. Completeness of the transcription process. Journal of Virology 10:689–697
    [Google Scholar]
  2. Cash P., Pringle C. R., Preston C. M. 1979; The polypeptides of human respiratory syncytial virus: products of cell-free protein synthesis and post-translational modifications. Virology 92:375–384
    [Google Scholar]
  3. Chanock R. M., Kapikian A. Z., Mills J., Kim H. W., Parrott R. H. 1970; Influence of immunological factors in respiratory syncytial virus disease of the lower respiratory tract. Archives of Environmental Health 21:347–355
    [Google Scholar]
  4. Choppin P. W., Compans R. W. 1975; The structure of influenza virus. In The Influenza Viruses and Influenza pp 15–51 Edited by Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  5. Elango N., Venkatesan S. 1983; Amino acid sequence of human respiratory syncytial virus nucleocapsid protein. Nucleic Acids Research 11:5941–5951
    [Google Scholar]
  6. Fernie B. F., Ford E. C., Gerin J. L. 1981; The development of Balb/c cells persistently infected with respiratory syncytial virus: presence of ribonucleoprotein on the cell surface. Proceedings of the Society for Experimental Biology and Medicine VSl83–86
    [Google Scholar]
  7. Gardner P. S. 1973; Respiratory syncytial virus infections. Postgraduate Medical Journal 49:788–791
    [Google Scholar]
  8. Konigsberg W. H., Steinman H. M. 1977; Strategy and methods of sequence analysis. In The Proteins vol 3 pp 1–178 Edited by Neurath H., Hill R. L. New York: Academic Press;
    [Google Scholar]
  9. Lambden P. R. 1982; Biochemical comparison of pili from variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 128:2105–2111
    [Google Scholar]
  10. Ogilvie M. M., Vathenen A. S., Radford M., Codd J., Key S. 1981; Maternal antibody and respiratory syncytial virus infection in infancy. Journal of Medical Virology 7:263–271
    [Google Scholar]
  11. Parrott R. H., Kim H. W., Arrobio J. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington DC. II. Infection and diseases with respect to age, immunologic status, race and sex. American Journal of Epidemiology 98:289–300
    [Google Scholar]
  12. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354
    [Google Scholar]
  13. Townsend A. R. M., Skehel J. J. 1982; Influenza A cytotoxic T-cell clones that do not recognize viral glycoproteins. Nature, London 300:655–657
    [Google Scholar]
  14. Van Wyke K. L., Hinshaw V. S., Bean W. J., Webster R. G. 1980; Antigen variation of influenza A virus nucleoprotein detected with monoclonal antibodies. Journal of Virology 35:24–30
    [Google Scholar]
  15. Walsh E. E., Hruska I. 1983; Monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. Journal of Virology 41:171–177
    [Google Scholar]
  16. Ward K. A., Lambden P. R., Ogilvie M. M., Watt P. J. 1983; Antibodies to respiratory syncytial virus polypeptides and their significance in human infection. Journal of General Virology 64:1867–1876
    [Google Scholar]
  17. Watanabe M., Ishii T., Nariuchi H. 1981; Fractionation of IgGl, IgG2a, IgG2b and IgG3 immunoglobulins from mouse serum by protein A-Sepharose column chromatography. Japanese Journal of Experimental Medicine 51:65–70
    [Google Scholar]
  18. Wunner W. H., Pringle C. R. 1976; Respiratory syncytial virus proteins. Virology 73:228–243
    [Google Scholar]
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