The susceptibility of freshly isolated tobacco protoplasts to infection by brome mosaic virus (BMV) fell rapidly to a low residual level over a period of 8 h in culture. In contrast, susceptibility to infection by cowpea chlorotic mottle virus (CCMV) fell much more slowly over 15 h in culture to a similar residual level. In the absence of polycations, BMV infected many more protoplasts if CCMV were also present in the inoculum even though CCMV did not infect such doubly inoculated protoplasts. Both effects are thought to be a consequence of the different electrical charges of particles of the viruses and support the view that inoculation of protoplasts depends primarily on physical interactions between virus and protoplast.


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